Citation: LI Lu, LIU Fei, CHEN Honghan, QIN Xiaopeng. Simultaneous determination of ciprofloxacin and flumequine in water samples by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2013, 31(6): 567-571. doi: 10.3724/SP.J.1123.2012.12052 shu

Simultaneous determination of ciprofloxacin and flumequine in water samples by high performance liquid chromatography

1.
Beijing Key Laboratory of Water Resource & Environmental Engineering, China University of Geosciences (Beijing), Beijing 100083, China

Corresponding author: LIU Fei,
Received Date: 28 December 2012
Available Online: 31 January 2013
Fund Project: 国家自然科学基金项目(51078338). (51078338)

In order to meet the field sample measurements and laboratory research, a simple, stable, and popular high performance liquid chromatographic (HPLC) method was established for the determination of the concentrations of two fluoroquinolone antibiotics in water samples simultaneously and rapidly. Thus, it could provide valuable information for other scholars to study the interactions between different antibiotics. Ciprofloxacin (CIP) and flumequine (FLU) were taken as the target contaminants because of their wide application in medical career. Furthermore, this study also investigated the different types of mobile phase, different mobile phase ratios, common ions (Ca²⁺, Mg²⁺, Fe³⁺, Al³⁺, SO₄^2-and HCO₃^-) in water samples and other factors on CIP and FLU measurements with this method. The results showed that triethylamine has obvious effect on improving column efficiency. Low concentrations of ions had little effect on the test, but Fe³⁺and Al³⁺might cause baseline instability, because Fe³⁺and Al³⁺may form complexes with the surface hydroxyl groups of the stationary phase or the test components. The results may be referred by other workers for the optimization of the determination conditions.
- high performance liquid chromatography (HPLC),
- fluoroquinolones,
- ciprofloxacin,
- flumequine,
- water samples
1. [1]
  [1] Xue Z S. China Journal of Pharmaceutical Economics (薛周山. 中国药物经济学), 2012(4): 117
2. [2]
  [2] Picó Y, Andreu V. Anal Bioanal Chem, 2007, 387(4): 1287
3. [3]
  [3] Turiel E, Esteban A M, Tadeo J L. Anal Chim Acta, 2006, 562: 30
4. [4]
  [4] Wang R, Liu T Z, Wang T. Acta Ecologica Sinica (王冉, 刘铁铮, 王恬. 生态学报), 2006, 26(1): 265
5. [5]
  [5] Mackay A A, Seremet D E. Environ Sci Technol, 2008, 42: 8270
6. [6]
  [6] Tan J H, Tang C M, Yu Y Y. Chinese Journal of Chromatography (谭建华, 唐才明, 余以义, 等. 色谱), 2007, 25(4): 546
7. [7]
  [7] Zhang H C, Huang C H. Chemosphere, 2007, 66: 1502
8. [8]
  [8] Zhou W J, Xie Z F, Shao L Z. Chinese Journal of Chromatography (周卫军, 谢正福, 邵琳智. 色谱), 2012, 30(7): 684
9. [9]
  [9] Ciprofloxacin//British Pharmacopoeia Volume I & II. Monographs: Medicinal and Pharmaceutical Substances. [2012-10-15]. http://www4.drugfuture.com/Pharmacopoeia/BP2010/data/7589.html
10. [10]
  [10] SPARC On-line Calculator. [2012-10-10]. http://ibmlc2.chem.uga.edu/sparc/
11. [11]
  [11] Sun Y Q, Hu Y Z. Selection and Optimization of LC Solvent System. Beijing: Chemical Industry Press (孙毓庆, 胡育筑. 液相色谱溶剂系统的选择与优化. 北京: 化学工业出版社), 2007: 4
12. [12]
  [12] Li X Y, Gui J Y, Zhang L, et al. Rock and Mineral Analysis (李晓亚, 桂建业, 张莉, 等. 岩矿测试), 2011, 30(3): 349
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