Citation: YU Xinwei, WU Qian, LV Wang, WANG Yan, MA Xiaoqiong, CHEN Zhe, YAN Chao. Metabonomics study of lung cancer cells based on liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(7): 691-696. doi: 10.3724/SP.J.1123.2012.12039 shu

Metabonomics study of lung cancer cells based on liquid chromatography-mass spectrometry

YU Xinwei ¹ ,
WU Qian ¹ ,
LV Wang ² ,
WANG Yan ¹ ,
MA Xiaoqiong ² ,
CHEN Zhe ^2,, ,
YAN Chao ^1,,

1.
1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;

Corresponding author: CHEN Zhe, YAN Chao,
Received Date: 15 December 2012

Fund Project: 国家自然科学基金项目(21175092,21105064) (21175092,21105064)国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) (2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210)浙江省科技厅公益性技术应用研究计划(2011C37014) (2011C37014)

The metabolic profiles of the polar metabolites and the non-polar metabolites in lung tumor cell lines H358, A549, HCC827, H1299, Calu-3, Calu-1, PC-9 and normal cell line MRC-5 were analyzed separately using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS). Partial least square discriminant analysis (PLS-DA) was used to process the metabolic data. The results showed that the metabolites of the lung cancer cell lines and normal cell line have significant differences. Further, 10 polar metabolites and 21 non-polar metabolites which had a significant contribution to classification were selected and preliminarily identified due to the accurate mass. Comparing with the normal cell line, the lung tumor cell lines present an abnormal metabolism in protein, fatty acid, and phospholipids. These results may provide important information for the early diagnosis of lung cancer.
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沈阳化工大学材料科学与工程学院沈阳 110142