Citation: ZHANG Xiaohui, WANG Rong, XIE Hua, YIN Qiang, LI Xiaoyun, JIA Zhengping, WU Xiaoyu, ZHANG Juanhong, LI Wenbin. Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride[J]. Chinese Journal of Chromatography, ;2013, 31(5): 451-455. doi: 10.3724/SP.J.1123.2012.11021 shu

Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

ZHANG Xiaohui ¹ ,
WANG Rong ^1,2,, ,
XIE Hua ¹ ,
YIN Qiang ¹ ,
LI Xiaoyun ¹ ,
JIA Zhengping ^1,2,, ,
WU Xiaoyu ¹ ,
ZHANG Juanhong ¹ ,
LI Wenbin ¹

1.
1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China;

Corresponding author: WANG Rong, JIA Zhengping,
Received Date: 28 November 2012
Available Online: 14 January 2013
Fund Project: 国家科技部重大资助项目(2008ZXJ09014-010) (2008ZXJ09014-010)全军"十二五"医药科研重点项目(BWS12J012) (BWS12J012)全军"十二五"医药科研面上项目(CWS11C231). (CWS11C231)

The online enrichment ability of the restricted-access media (RAM) column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride in plasma was studied. The RAM-HPLC system consisted of an RAM column as enrichment column and a C18 column as analytical column coupled via the column switching technique. The effects of the injection volume on the peak area and the systematic pressure were studied. When the injection volume was less than 100 μL, the peak area increased with the increase of the injection volume. However, when the injection volume was more than 80 μL, the pressure of whole system increased obviously. In order to protect the whole system, 80 μL was chosen as the maximum injection volume. The peak areas of ordinary injection and the large volume injection showed a good linear relationship. The enrichment ability of RAM-HPLC system was satisfactory. The system was successfully used for the separation and detection of the trace benazepril hydrochloride in rat plasma after its administration. The sensitivity of HPLC can be improved by RAM pre-enrichment. It is a simple and economic measurement method.
- restricted-access media (RAM),
- high-performance liquid chromatography (HPLC),
- online enrichment,
- direct injection,
- benazepril hydrochloride,
- serum
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