Citation: ZENG Kai, LIU Zhirong, NING Yajun, LI Jinlin, TANG Jun, ZHANG Min. Simultaneous determination of melamine and creatinine in milk and milk products by gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(5): 477-480. doi: 10.3724/SP.J.1123.2012.11008 shu

Simultaneous determination of melamine and creatinine in milk and milk products by gas chromatography-mass spectrometry

ZENG Kai ^1,, ,
LIU Zhirong ² ,
NING Yajun ¹ ,
LI Jinlin ¹ ,
TANG Jun ¹ ,
ZHANG Min ¹

1.
1. Nanchang Food Quality Health Safety Supervision and Inspection Center, Nanchang 330012, China;

Corresponding author: ZENG Kai,
Received Date: 19 November 2012
Available Online: 23 January 2013
Fund Project: 江西省质量技术监督局科技项目(GZJK201102). (GZJK201102)

A method established for determining melamine and creatinine in milk and milk products. The samples were extracted with 1% trichloracetic acid solution. The extract was cleaned-up by using a mixed-mode cation ion exchange SPE column and evaporated to dryness under nitrogen. The residue was derived by bis(trimethylsilyl)trifluoroacetamide-chlorotrimethylsilane (BSTFA-TMCS), then determined by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. Melamine and creatinine had good linearity in the range of 0.1-50 mg/L with the correlation coefficients higher than 0.99, and the limits of quantification were 0.10 mg/kg and 0.20 mg/kg, respectively. The recoveries of melamine and creatinine were 80.7%-116.8% and 77.6%-107.5% with RSD no more than 9.4% and 8.5% in the spiked range of 10-100 mg/kg and 0.1-5.0 mg/kg, respectively. The method is accurate, sensitive, and suitable for the determination of creatinine in milk and milk products.
- gas chromatography-mass spectrometry (GC-MS),
- melamine,
- creatinine,
- milk,
- milk products
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