Citation: WEN Quan, LIU Hong-Ling, SU Guan-Yong, WEI Si, FENG Jian-Fang, YU Hong-Xia. Determination of Polybrominated Diphenyl Ethers and Their Derivate in Zebrafish Eggs[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1698-1702. doi: 10.3724/SP.J.1096.2012.20361 shu

Determination of Polybrominated Diphenyl Ethers and Their Derivate in Zebrafish Eggs

1.
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210046, China

Corresponding author: YU Hong-Xia,
Received Date: 26 April 2012
Available Online: 13 July 2012
Fund Project: 本文系国家自然科学基金项目(Nos.20977047, 20737001)资助 (Nos.20977047, 20737001)

An analytical method was developed for the simultaneous analysis of 10 kinds of PBDEs, 12 MeO-PBDEs and 9 kinds of HO-PBDEs in zebrafish eggs by liquid-liquid extraction coupled with GC/MS/MS. The conditions of extraction, chromatography and mass spectrometry were optimized. The target compounds were extracted through liquid-liquid extraction and derivatization of HO-PBDEs can be carried out without separation from extraction solution. Thus the pre-treatment time could be shortened and all target compounds could be analyzed simultaneously. Purification was conducted by dry packed acidic silica gel column. All compounds can be eluted with 15mL n-hexane and 15mL of dichloromethane and hexane (1:1, V/V) mixed solvent. Under optimized conditions, 31 compounds had good linearity in concentration range from 4.0 to 500 μg/L, with correlation coefficient (R²) better than 0.9933, recoveries in the range of 71.6%-133.0% with relative standard deviations less than 21.5%, and the method and instrument detection limits varied from 0.09 to 17.0 ng/g. The presented method was applied for maternal transfer study of BDE-47, 6-HO-BDE-47, 6-MeO-BDE-47, 2'-HO-BDE-28 and 2'-MeO-BDE-28 and turned out to be efficient for real sample analysis. After maternal exposure, the five compounds showed different maternal transfer potency in zebrafish, giving new consideration for the exposure assessment of such compounds.
- Liquid-liquid extraction,
- Pre-column derivatization method,
- Zebrafish eggs,
- Maternal transfer
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沈阳化工大学材料科学与工程学院沈阳 110142