Citation: LI Chun-Zheng, CHEN Jia, ZHONG Yu-Huan, ZHONG Yu-Xu, XIE Jian-Wei, LI Hua. Simultaneous Quantification of Thioglycol and Thioglycol Sulfoxide in Rat Plasma by Isotope Dilution-Liquid Chromatography Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1567-1572. doi: 10.3724/SP.J.1096.2012.20249 shu

Simultaneous Quantification of Thioglycol and Thioglycol Sulfoxide in Rat Plasma by Isotope Dilution-Liquid Chromatography Tandem Mass Spectrometry

1.
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China

Corresponding author: LI Hua,
Received Date: 12 March 2012
Available Online: 15 May 2012
Fund Project: 本工作系国家"重大新药创制"科技重大专项(2008ZXJ09006-001,2010ZXJ0900X-003-002)资助 (2008ZXJ09006-001,2010ZXJ0900X-003-002)

A method based on isotope dilution-liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was developed and validated to simultaneously quantify metabolites of sulfur mustard, thioglycol (TDG) and thioglycol sulfoxide (TDGO), in rat plasma. Plasma samples were pretreated with the mixed solvent of methanol and acetonitrile to precipitate proteins. The separation of TDG and TDGO was achieved on a ZORBAX-C₁₈ column (3.0 mm×100 mm, 3.5 μm) by gradient elution with mobile phase consisting of methanol and 5 mmol/L ammonium formate aqueous solution. The mass spectrometric identification and quantification were performed using positive electrospray ionization and multiple reactions monitoring mode. An isotopic labeled TDG (d₈-TDG) was used as internal standard. The calibration curves for TDG and TDGO were linear (R²＞0.991) over the range from 5-800 μg/L, and 0.5-80 μg/L, with the lower limit of quantification at 5 and 0.5 μg/L. The recovery of the analytes ranged from 101% to 118%. The intra- and inter-day precisions (RSD) were all within 10%. The plasma was collected and analyzed from HD-exposure rats after subcutaneous administration, and the kinetics parameters of TDG and TDGO were calculated and demonstrated as follow: t_max 30 min and 60 min, c_max (1724±227) μg/L and (301±115) μg/L, AUC (3286±249) μg·h/L and (1010±363) μg·h/L, respectively.
- Sulfur mustard,
- Thioglycol,
- Thioglycol sulfoxide,
- Liquid chromatography-tandem mass spectrometry,
- Plasma,
- Toxicokinetics
1. [1]
  1 Takafuji E T, Franz D R. Medical Aspects of Chemical and Biological Warfare, Office of The Surgeon General, Washington D.C., TMM Publications, 1997: 198-200
2. [2]
  2 Conference on Disarmament. The Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, United Nations Document CD/ 1170, Geneva, Switzerland, 1992
3. [3]
  3 Black R M, Read R W. J. Chromatogr. B, 1995, 665(1): 97-105
4. [4]
  4 US Army Medical Research Institute of Chemical Defense (USAMRICD). Medical Management of Chemical Casualties Handbook. 4th Ed., Aberdeen Proving Ground, MD, USA, 2007: 83
5. [5]
  5 Mesilaakso, M. Chemical Weapons Convention Chemicals Analysis: Sample Collection, Preparation and Analytical Methods; 1st Ed.; Chichester: John Wiley & Sons, 2005: 409-416
6. [6]
  6 Capacio B R, Smith J R, DeLion M T, Anderson D R, Graham J S, Platoff G E, Korte W D. J. Anal. Toxicol, 2004, 28(5): 306-310
7. [7]
  7 Jakubowski E M, Sidell F R, Evans R A, Carter M A, Keeler J R, Evans R A, McMonagle J D. Toxicol Mechanism Methods, 2000, 10(2): 143-150
8. [8]
  8 Noort D, Fidder A, Benschop H P, de Jong L P A, Smith J R J. Anal. Toxicol., 2004, 28(5): 311-315
9. [9]
  9 Smith J R, Capacio B R, Korte W D J. Anal. Toxicol., 2008, 32(1): 17-24
10. [10]
  10 U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Center for Veterinary Medicine (CVM), Guidance for Industry, Bioanalytical Method Validation, 2001
1. [1]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
2. [2]
  Jiageng Li , Putrama . 数值积分耦合非线性最小二乘法一步确定反应动力学参数. University Chemistry, 2025, 40(6): 364-370. doi: 10.12461/PKU.DXHX202407098
3. [3]
  Jichao XU , Ming HU , Xichang CHEN , Chunhui WANG , Leichen WANG , Lingyi ZHOU , Xing HE , Xiamin CHENG , Su JING . Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1495-1504. doi: 10.11862/CJIC.20250144
4. [4]
  Nan Xiao , Fang Sun . 二芳基硫醚化合物的构建及应用. University Chemistry, 2025, 40(6): 360-363. doi: 10.12461/PKU.DXHX202407099
5. [5]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
6. [6]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
7. [7]
  Jiayu Gu , Siqi Wang , Jun Ling . Kinetics of Living Copolymerization: A Brief Discussion. University Chemistry, 2025, 40(4): 100-107. doi: 10.12461/PKU.DXHX202406012
8. [8]
  Jinfu Ma , Hui Lu , Jiandong Wu , Zhongli Zou . Teaching Design of Electrochemical Principles Course Based on “Cognitive Laws”: Kinetics of Electron Transfer Steps. University Chemistry, 2024, 39(3): 174-177. doi: 10.3866/PKU.DXHX202309052
9. [9]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
10. [10]
  Wenwen Zhang , Peichao Zhang , Conghao Gai , Xiaoyun Chai , Yan Zou , Qingjie Zhao . Unveiling Kinetics at Natural Abundance: ¹³C NMR Isotope Effect Experiments. University Chemistry, 2025, 40(10): 203-207. doi: 10.12461/PKU.DXHX202411076
11. [11]
  Haozhe Hu , Haoyu Zhang , Changsheng Lu . Study on the Precipitation Process of Elemental Sulfur from the Decomposition Products of Thiosulfuric Acid: Is It an Unexpected Failed Experiment?. University Chemistry, 2025, 40(11): 409-415. doi: 10.12461/PKU.DXHX202412034
12. [12]
  Cuicui Yang , Bo Shang , Xiaohua Chen , Weiquan Tian . Understanding the Wave-Particle Duality and Quantization of Confined Particles Starting from Classic Mechanics. University Chemistry, 2025, 40(3): 408-414. doi: 10.12461/PKU.DXHX202407066
13. [13]
  Xuzhen Wang , Xinkui Wang , Dongxu Tian , Wei Liu . Enhancing the Comprehensive Quality and Innovation Abilities of Graduate Students through a “Student-Centered, Dual Integration and Dual Drive” Teaching Model: A Case Study in the Course of Chemical Reaction Kinetics. University Chemistry, 2024, 39(6): 160-165. doi: 10.3866/PKU.DXHX202401074
14. [14]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
15. [15]
  Jiajie Cai , Chang Cheng , Bowen Liu , Jianjun Zhang , Chuanjia Jiang , Bei Cheng . CdS/DBTSO-BDTO S-scheme photocatalyst for H₂ production and its charge transfer dynamics. Acta Physico-Chimica Sinica, 2025, 41(8): 100084-0. doi: 10.1016/j.actphy.2025.100084
16. [16]
  Shanghua Li , Malin Li , Xiwen Chi , Xin Yin , Zhaodi Luo , Jihong Yu . High-Stable Aqueous Zinc Metal Anodes Enabled by an Oriented ZnQ Zeolite Protective Layer with Facile Ion Migration Kinetics. Acta Physico-Chimica Sinica, 2025, 41(1): 100003-0. doi: 10.3866/PKU.WHXB202309003
17. [17]
  Linlin Wu , Yonghua Zhou , Zhongbei Li , Liu Deng , Younian Liu , Limiao Chen , Jianhan Huang . Digital Education Promoting Applied Chemistry Comprehensive Experiments: A Case Study of Catalytic Oxidation of Hydrogen Chloride and Reaction Kinetics. University Chemistry, 2025, 40(9): 273-278. doi: 10.12461/PKU.DXHX202411018
18. [18]
  Xinyu Xu , Jiale Lu , Bo Su , Jiayi Chen , Xiong Chen , Sibo Wang . Steering charge dynamics and surface reactivity for photocatalytic selective methane oxidation to ethane over Au/Ti-CeO₂. Acta Physico-Chimica Sinica, 2025, 41(11): 100153-0. doi: 10.1016/j.actphy.2025.100153
19. [19]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
20. [20]
  Yan'e LIU , Shengli JIA , Yifan JIANG , Qinghua ZHAO , Yi LI , Xinshu CHANG . MoO₃/cellulose derived carbon aerogel: Fabrication and performance as cathode for lithium-sulfur battery. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1565-1573. doi: 10.11862/CJIC.20250054

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(667)
HTML views(29)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142