Citation: ZHAO Yan-Hua, LIN Ni-Ni, GUO Lei, CHEN Jia, LIU Wan-Hui, XIE Jian-Wei. Simultaneous Determination of T-2 Toxin and Its Metabolites in Rat Plasma Using Solid Phase Extraction and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1852-1858. doi: 10.3724/SP.J.1096.2012.20124 shu

Simultaneous Determination of T-2 Toxin and Its Metabolites in Rat Plasma Using Solid Phase Extraction and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHAO Yan-Hua ^1,2 ,
LIN Ni-Ni ¹ ,
GUO Lei ¹ ,
CHEN Jia ¹ ,
LIU Wan-Hui ² ,
XIE Jian-Wei ^1,,

1.
¹ College of Pharmacy, Yantai University, Yantai 264005, China;

Corresponding author: XIE Jian-Wei,
Received Date: 15 March 2012
Available Online: 22 May 2012
Fund Project: 本文系"十二五"国家科技支撑计划(No.2011BAK10B07)资助项目 (No.2011BAK10B07)

T-2 toxin and its 5 metabolites in rat plasma were simultaneously determined by an ultra performance liquid chromatography coupled with triple quadrupole-ion trap mass spectrometric (UPLC-QTRAP) method with high sensitivity. In the optimized solid phase extraction conditions, the rat plasma samples were purified and concentrated by a HLB column, and then the analytes were separated on a XDB-C18 column (4.6 mm×50 mm, 1.8 μm) with a mobile phase of methanol and aqueous solution containing 5 mmol/L ammonium acetate under a gradient elution. The mass spectrometric identification and quantification were performed with electrospray ionization in a positive by using multiple reaction monitoring mode with zearalanone as internal standard. In this established method, good linearities (R²>0.997) in the range from 0.1 to 500 μg/L with the limits of detection between 0.02 to 0.3 μg/L, and the recoveries varied from 93.5% to 120.3% with a relative standard deviations less than 13% were obtained. The method is rapid, reproducible, sensitive, and can be applied in the retrospective determination of the intoxication event of T-2 toxin.
- T-2 toxin,
- Metabolites,
- Solid phase extraction,
- Ultra performance liquid chromatography tandem mass spectrometry,
- Plasma
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