Citation: WANG Yan, WU Bin, ZHANG Xiao-Yan, SHEN Chong-Yu, ZHANG Rui, LI Li-Hua, FEI Xiao-Qing, ZHAO Zeng-Yun. Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1602-1606. doi: 10.3724/SP.J.1096.2012.20111 shu

Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector

1.
Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China

Corresponding author: WANG Yan,
Received Date: 10 February 2012
Available Online: 18 April 2012

A new method for the determination of detecting β-fructofruanosidase in honey was developed by liquid chromatography with refractive index detector (HPLC-RID). The method was based on the changes of product concentration of melibiose by the reaction of β-fructofruanosidase enzyme with raffinose in the medium of citric acid-sodium hydroxide solution (pH＝4.50) for 16 h in water bath at 50℃. Agilent Carbohydrate was chosen as the separation column, acetonitrile-water (78:22, V/V) was selected as mobile phase with flow rate of 1.4 mL/min. The column temperature and detector temperature was 35℃. The results showed that the numbers of peaks were simplified and the sensitivity of this proposed method was improved 1.3 fold as well by using polyacrylamide gel column for pre-separation. A good linear fit was received for the concentration of β-fructofruanosidase enzyme from 10 U/kg to 200 U/kg with the detection limit of 10 U/kg. Recovery was obtained between 81.3% and 112.4% with the relative standard deviation of 3.7% to 9.7% (n＝6). It is judged to be adulteration honey if β-fructofruanosidase concentration is more than 20 U/kg,
- β-Fructofruanosidase,
- Honey,
- Refractive index detector,
- Liquid chromatography,
- Adulteration
1. [1]
  1 TU Zhen-Hua, ZHU Da-Zhou, JI Bao-Ping, MENG Chao-Ying, WANG Lin-Ke, QING Zhao-Kun. Spectroscopy and Spectral Analysis, 2010, 30(11):2971-2975
2. [2]
  屠振华, 朱大洲, 籍保平, 孟超英, 王林舸, 庆兆坤. 光谱学与光谱分析, 2010, 30(11):2971-2975
3. [3]
  2 YI Song-Qiang. Research on β-glucosidase (BGL) in Honey, Paper of Master Degree of Zhejiang University. 2011
4. [4]
  易松强. 蜂蜜中β-葡萄糖苷酶的研究, 浙江大学硕士学位论文, 2011
5. [5]
  3 AOAC Official Method 998.12, C-4 Plant sugars in Honey Internal Standard Stable Carbon Isotope Ratio Method Fist Action 1998, AOAC standard
6. [6]
  4 AOAC Official Method 991.41, C-4 Plant Sugars in Honey Internal Standard Stable Carbon Isotope Ratio Method Fist Action 1991 Final Action 1996, AOAC Standard
7. [7]
  5 GB/T 18932.1-2002. Method for the Determination of C-4 Plant Sugars in Honey-Stable Carbon Isotope Ratio Method. National Standards of the People's Republic of China
8. [8]
  6 GB/T 18932.22-2003, Method for the Determination of Fructose, Glucose, Sucrose, Maltose Contents in Honey—Liquid Chromatography Refractive Index Detection Method, National Standards of the People's Republic of China
9. [9]
  GB/T 18932.22-2003, 蜂蜜中果糖、葡萄糖、蔗糖、麦芽糖含量的测定方法液相色谱示差折光检测法, 中华人民共和国国家标准
10. [10]
  7 GB/T 18932.2-2002, Method for the Determination of High Fructose Starch Syrup in Honey-Thin-Layer Chromatographic Method, National Standards of the People's Republic of China.
11. [11]
  GB/T 18932.2-2002, 蜂蜜中高果淀粉糖浆测定方法薄层色谱法, 中华人民共和国国家标准
12. [12]
  8 Irudayaraj J, Xu F, Tewar J. Journal of Food Science, 2003, 68(6):2040-2045
13. [13]
  9 Krummen M, Hilkert A W, Juchelka D, Duhr A, Schlüter H J, Pesch R. Rapid Communications in Mass Spectrometry, 2004, 18(19):2260-2266
14. [14]
  10 Cabaero Ana I, Recio Jose L, Rupérez M. Journal of Agricultural and Food Chemistry, 2006, 54(26):9719-9727
15. [15]
  11 Elflein L, Raezke K P. Apidologie, 2008, 39(5):574-587
16. [16]
  12 Boschker H T S, Moerdijk-Poortvliet T C W, van Breugel P, Houtekamer M, Middelburg J. J. Rapid Communications in Mass Spectrom, 2008, 22(23):3902-3908
17. [17]
  13 FEI Xiao-Qing, WU Bin, SHEN Chong-Yu, DING Tao, LI Li-Hua, LU Ying. Chinese Journal of Chromatography, 2011, 29(1):15-19
18. [18]
  费晓庆, 吴斌, 沈崇钰, 丁涛, 李丽花, 路颖. 色谱, 2011, 29(1):15-19
19. [19]
  14 Nguyen Q D, Rezessy-Szabó J M, Bhat M K, goston H. Progress Biochemistry, 2005, 40(7):2461-2466
1. [1]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
4. [4]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
7. [7]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
8. [8]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
9. [9]
  Xiao Liu , Guangzhong Cao , Mingli Gao , Hong Wu , Hongyan Feng , Chenxiao Jiang , Tongwen Xu . Seawater Salinity Gradient Energy’s Job Application in the Field of Membranes. University Chemistry, 2024, 39(9): 279-282. doi: 10.3866/PKU.DXHX202306043
10. [10]
  Jian Li , Yu Zhang , Rongrong Yan , Kaiyuan Sun , Xiaoqing Liu , Zishang Liang , Yinan Jiao , Hui Bu , Xin Chen , Jinjin Zhao , Jianlin Shi . Highly Efficient, Targeted, and Traceable Perovskite Nanocrystals for Photoelectrocatalytic Oncotherapy. Acta Physico-Chimica Sinica, 2025, 41(5): 100042-0. doi: 10.1016/j.actphy.2024.100042
11. [11]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
12. [12]
  Hao Ren , Wen Zhao , Fangna Dai , Wenyue Guo . Finite Difference Solution of One-Dimensional Quantum Systems: (1) Fundamental Concepts and Infinite Square Well. University Chemistry, 2025, 40(3): 124-131. doi: 10.12461/PKU.DXHX202405145
13. [13]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
14. [14]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
15. [15]
  Donghui PAN , Yuping XU , Xinyu WANG , Lizhen WANG , Junjie YAN , Dongjian SHI , Min YANG , Mingqing CHEN . Preparation and in vivo tracing of ⁶⁸Ga-labeled PM_2.5 mimetic particles for positron emission tomography imaging. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 669-676. doi: 10.11862/CJIC.20230468
16. [16]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
17. [17]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
18. [18]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
19. [19]
  Jiandong Liu , Xin Li , Daxiong Wu , Huaping Wang , Junda Huang , Jianmin Ma . Anion-Acceptor Electrolyte Additive Strategy for Optimizing Electrolyte Solvation Characteristics and Electrode Electrolyte Interphases for Li||NCM811 Battery. Acta Physico-Chimica Sinica, 2024, 40(6): 2306039-0. doi: 10.3866/PKU.WHXB202306039
20. [20]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1258)
HTML views(151)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142