Citation: SHI Jing-Jing, LIAN Jie, ZHOU Wen-Wen, SHI Xi-Zeng, GAO Yun-Hua. Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1524-1529. doi: 10.3724/SP.J.1096.2012.20046 shu

Magnetoresistive Biosensor for Detection of Chloramphenicol Residue in Milk

1.
¹ Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;

Corresponding author: GAO Yun-Hua,
Received Date: 13 January 2012
Available Online: 18 April 2012
Fund Project: 本文系国家自然科学基金(No.20975106) (No.20975106) 中国科学院知识创新工程重要方向项目(No.KJCX2-YW-M15) (No.KJCX2-YW-M15)广东省中国科学院全面战略合作项目(No.2011A090100036)资助 (No.2011A090100036)

Based on direct competitive immunoassay principle and giant magnetoresistance (GMR) effect, a novel method for detecting chloramphenicol (CAP) by magnetic biosensor has been developed. The sample, biotinylated anti-CAP monoclonal antibody, and streptavidin-beads conjugate were added onto the prepared CAP hapten chips one by one. Subsequently, competitive immune reaction happened and the number of beads bonded on chips was detected by magnetic biosensor. The standard curve was established by optimizing the detection condition. This method can be used to detect CAP in a range of 0.05-100.0 μg/L, detection limit of 50 ng/L. The analyte recoveries in milk samples ranged from 95.97% to 99.36%. The relative standard deviations ranged from 0.8% to 3.9% for intra-assay and 1.1% to 1.7% for inter-assay. The correlation coefficient between this method and ELISA was 0.98. Rapid quantitative detection could be accomplished within 30 minutes using this method. It provides feasibility for the establishment of rapid multi-target magnetic competitive immunoassay system.
- Magnetic competitive immunoassay,
- Magnetic biosensor,
- Chloramphenicol,
- Number of beads
1. [1]
  1 Maciej J B, Huda H, Eid A E, Zuhour I, Mohammed A T. J. Chromatogr. B, 2004, 807(2): 343-356
2. [2]
  2 Vivekanandan K, Guru S M, Prasad S. Rapid Commun. Mass Spectrom., 2005, 19(21): 3025-3030
3. [3]
  3 Maximum Residue Limit of Veterinary Drug in Animal-Source Food. Attachment of Announcement No.235, Ministry of Agriculture of the People's Republic of China.
4. [4]
  动物源性食品中兽药最高残留限量. 中华人民共和国农业部第235号公告附件
5. [5]
  4 WANG Shi-Cheng, LI Guo-Chen, WANG Yan-Hong, HU Xiao-Yan, ZHANG Hong. Chinese J.Anal.Chem., 2010, 38(8): 1199-1202
6. [6]
  王世成, 李国琛, 王颜红, 胡小燕, 张红. 分析化学, 2010, 38(8): 1199-1202
7. [7]
  5 Epstein R L, Henry C, Holland K P, Dreas J. J. AOAC Int., 1994, 77: 570-576
8. [8]
  6 Brner S, Fry H, Balizs G, Kroker R. J. AOAC Int., 1995, 78: 1153-1160
9. [9]
  7 van Ginkel L A, van Rossum H J, Zoontjes P W, van Blitterswijk H, Ellen G, Vander Heft E, Zomer G. Anal. Chim. Acta, 1990, 237: 61-69
10. [10]
  8 Shen H Y, Jiang H L. Anal. Chim. Acta, 2005, 535(1-2): 33-41
11. [11]
  9 Byzova N A, Zvereva E A, Zherdeva A V, Ereminb S A, Dzantiev B B. Talanta, 2010, 81(3): 843-848
12. [12]
  10 Kima D M, Rahmanb M A, Doa M H, Banc C, Shima Y B. Biosen. Bioelectron., 2010, 25(7): 1781-1788
13. [13]
  11 Xu L, Yu H, Akhras M S, Han S J, Osterfeld S, White R L, Pourmand N, Wang S X. Biosen. Bioelectron., 2008, 24(1): 99-103
14. [14]
  12 GasterR S, Hall D A, Wang S X. Lab Chip, 2011, 11(5): 950-956
15. [15]
  13 WU Ling-Ling, JIANG Nan. Occupation and Health, 2008, 24(5): 428-429
16. [16]
  吴玲玲, 姜楠. 职业与健康, 2008, 24(5): 428-429
17. [17]
  14 LIU Nan, GAO Zhi-Xian, SU Pu, CHAO Fu-Huan, ZHU Mao-Xiang, YANG Zhi-Hua, PAN Xiu-Jie. Bull Acad. Mil. Med. Sci., 2009, 33(1): 41-44, 65
18. [18]
  刘楠, 高志贤, 苏璞, 晁福寰, 朱茂祥, 杨陟华, 潘秀颉. 军事医学科学院院刊, 2009, 33(1): 41-44, 65
19. [19]
  15 WU Hui-Juan, WEI Ling, LIU Qing-Jun, LE Jia-Chang, LI Bao-Ming, ZHANG Xiao-Li, GAO Li-Juan, KANG Zi-Jia, ZHAO Long, QIAN Jia-Lin. Food Science, 2010, 31(8): 167-170
20. [20]
  武会娟, 魏玲, 刘清珺, 乐加昌, 李宝明, 张小莉, 高丽娟, 亢子佳, 赵龙, 钱嘉林. 食品科学, 2010, 31(8): 167-170
1. [1]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
2. [2]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
3. [3]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
4. [4]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
5. [5]
  Lin′an CAO , Dengyue MA , Gang XU . Research advances in electrically conductive metal-organic frameworks-based electrochemical sensors. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 1953-1972. doi: 10.11862/CJIC.20250160
6. [6]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . Photomultiplication-Type All-Polymer Photodetectors and Their Applications in Photoplethysmography Sensor. Acta Physico-Chimica Sinica, 2025, 41(1): 100007-0. doi: 10.3866/PKU.WHXB202311021
7. [7]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-0. doi: 10.3866/PKU.WHXB202310046
8. [8]
  Peiling Li , Qing Feng , Hongling Yuan , Qin Wang . Live Interview Recording about the Penicillin Family. University Chemistry, 2024, 39(9): 122-127. doi: 10.3866/PKU.DXHX202311022
9. [9]
  Xiaojing Tian , Zhichun Huang , Qingsong Zhang , Xu Wang , Ning Yang , Nanping Deng . PNIPAm Thermo-Responsive Nanofibers Mats: Morphological Stability and Response Behavior under Cross-Linking. Acta Physico-Chimica Sinica, 2024, 40(4): 2304037-0. doi: 10.3866/PKU.WHXB202304037
10. [10]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
11. [11]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
12. [12]
  Ziliang KANG , Jiamin ZHANG , Hong AN , Xiaohua LIU , Yang CHEN , Jinping LI , Libo LI . Preparation and water adsorption properties of CaCl₂@MOF-808 in-situ composite moulded particles. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2133-2140. doi: 10.11862/CJIC.20240282
13. [13]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
14. [14]
  Zhuo WANG , Xiaotong LI , Zhipeng HU , Junqiao PAN . Three-dimensional porous carbon decorated with nano bismuth particles: Preparation and sodium storage properties. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 267-274. doi: 10.11862/CJIC.20240223
15. [15]
  Qin ZHU , Jiao MA , Zhihui QIAN , Yuxu LUO , Yujiao GUO , Mingwu XIANG , Xiaofang LIU , Ping NING , Junming GUO . Morphological evolution and electrochemical properties of cathode material LiAl_0.08Mn_1.92O₄ single crystal particles. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1549-1562. doi: 10.11862/CJIC.20240022
16. [16]
  Zelong LIANG , Shijia QIN , Pengfei GUO , Hang XU , Bin ZHAO . Synthesis and electrocatalytic CO₂ reduction performance of metal-organic framework catalysts loaded with silver particles. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 165-173. doi: 10.11862/CJIC.20240409
17. [17]
  Wenjun Yang , Qiaoling Tan , Wenjiao Xie , Xiaoyu Pan , Youyong Yuan . Construction and Characterization of Calcium Alginate Microparticle Drug Delivery System: A Novel Design and Teaching Practice in Polymer Experiments. University Chemistry, 2025, 40(3): 371-380. doi: 10.12461/PKU.DXHX202405150
18. [18]
  Bizhu Shao , Huijun Dong , Yunnan Gong , Jianhua Mei , Fengshi Cai , Jinbiao Liu , Dichang Zhong , Tongbu Lu . Metal-Organic Framework-Derived Nickel Nanoparticles for Efficient CO₂ Electroreduction in Wide Potential Windows. Acta Physico-Chimica Sinica, 2024, 40(4): 2305026-0. doi: 10.3866/PKU.WHXB202305026
19. [19]
  Chenye An , Sikandaier Abiduweili , Xue Guo , Yukun Zhu , Hua Tang , Dongjiang Yang . Hierarchical S-scheme Heterojunction of Red Phosphorus Nanoparticles Embedded Flower-like CeO₂ Triggering Efficient Photocatalytic Hydrogen Production. Acta Physico-Chimica Sinica, 2024, 40(11): 2405019-0. doi: 10.3866/PKU.WHXB202405019
20. [20]
  Yan Yuan , Haitao Wu , Yi Zhang , Li Jiang , Feng Cao , Yanmao Dong . Research on the Talent Training System to Enhance the Core Competence of Employment for Undergraduate Students Majoring in Materials Chemistry. University Chemistry, 2024, 39(11): 52-56. doi: 10.12461/PKU.DXHX202402015

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(508)
HTML views(8)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142