Citation: ZHANG Ming-Rui, HAN Xiao-Jun. Construction of Phospholipid Analogue Vesicle and Study of Its Artificial Cell Functions[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(5): 884-891. doi: 10.19756/j.issn.0253-3820.221630 shu

Construction of Phospholipid Analogue Vesicle and Study of Its Artificial Cell Functions

ZHANG Ming-Rui ,
HAN Xiao-Jun ^,

State Key Laboratory of Urban Water Resource, Environment School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China

Corresponding author: HAN Xiao-Jun, hanxiaojun@hit.edu.cn
Received Date: 21 December 2022
Revised Date: 15 February 2023

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 21929401, 22174031, 22111540252), the Natural Science Foundation of Heilongjiang Province (No. ZD2022B001) and the Heilongjiang Touyan Team Project (No. HITTY- 20190034).

Phospholipids, as natural components of cell membrane systems, are widely used to construct artificial cells. However, the synthesis and purification of natural phospholipids are complicated. Chemically synthesized phospholipid analogues are structurally similar to natural phospholipids, so they can be used to mimic phospholipids to construct artificial cells. In this work, a novel phospholipid analogue 2 was synthesized by the azide-alkyne click reaction, which could form vesicles. The membrane was fluid, which indicated the vesicles could mimic cell membranes. The vesicles were capable to encapsulate small molecule of calcein and macromolecule of rhodamine B isothiocyanate-dextran. Melittins were embedded in the membrane to form pores, which allowed the transmembrane transport of calcein. When the concentrations of melittin were 1.0, 2.0, 3.0, 4.0 and 5.0 μg/mL, the transmembrane diffusion coefficients of calcein were 0.33, 0.46, 0.70, 1.37 and 1.91 μm²/s, respectively. Glucose oxidase and horse radish peroxidase were encapsulated into vesicles to fabricate artificial cells, which mimicked the cell metabolism function. Amplex red was converted into resorufin via enzyme cascade reactions. The synthesized phospholipid analogues could provide new building blocks for artificial cells.
- Click chemistry,
- Artificial cells,
- Calcein,
- Melittin,
- Enzyme cascade reaction
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