Citation: WANG Hao-Dong, YAO Chao-Qun, FAN Ji-Wen, HE Yu-Quan, WANG Zhen-Xin. Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(6): 945-953. doi: 10.19756/j.issn.0253-3820.221536 shu

Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples

WANG Hao-Dong ¹ ,
YAO Chao-Qun ^2,3 ,
FAN Ji-Wen ¹ ,
HE Yu-Quan ^1,, ,
WANG Zhen-Xin ^2,3,,

1.
Department of Cardiovascular, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
3.
School of Applied Chemical Engineering, University of Science and Technology of China, Hefei 230026, China

Corresponding author: HE Yu-Quan, WANG Zhen-Xin,
Received Date: 1 November 2022
Revised Date: 3 December 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.21775145).

Cardiac troponin I (cTnI) is one of the standard biomarkers of myocardial injury, and the rapid detection of its concentration in serum is crucial for the diagnosis of acute myocardial infarction (AMI). In this study, gold nanorods (GNRs) modified with mouse anti-cTnI monoclonal antibody (4T21cc-19C7cc, dAb) were used as labeling probes to prepare lateral flow immunochromatographic test strips (LFITS) by double-antibody sandwich method for rapid detection of cTnI in clinical serum samples. The GNR-labeled LFITS (termed as GNRLFITS) showed several advantages including quantitative detection, high sensitivity and good specificity. The GNR-LFITS exhibited a linearity with the concentration of cTnI in the range of 5-100 ng/mL in serum, with a low limit of detection (LOD, 1.2 ng/mL) and low values of cross reaction with other protein interfering substances (<5%). Furthermore, the practicability of as-developed GNR-LFITS was demonstrated by quantitative determination of cTnI in clinical serum samples. The results of GNR-LFITS exhibited a good correlation with those of commercial enzyme-linked immunosorbent assay (ELISA) detection kit (R²=0.989), suggesting that GNR-LFITS had great potential in the early diagnosis of AMI.
- Gold nanorods,
- Immunochromatographic test strip,
- Cardiac troponin I,
- Acute myocardial infarction
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