Citation: WU Yi, SHAO Si-Meng, LI Meng-Yuan, WAN Xi-Lin, ZHANG Zhe, YANG Hong-Mei. Investigation of Interaction Between RCA120 and Ginseng Oligosaccharides by Ultra-High Performance Liquid Chromatography Coupled with Orbitrap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(1): 112-119. doi: 10.19756/j.issn.0253-3820.221420 shu

Investigation of Interaction Between RCA120 and Ginseng Oligosaccharides by Ultra-High Performance Liquid Chromatography Coupled with Orbitrap Mass Spectrometry

1.
Northeast Asia Institute of Chinese Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China;
2.
Jilin Ginseng Academy, Changchun University of Traditional Chinese Medicine, Changchun 130117, China

Corresponding author: YANG Hong-Mei, yanghm0327@sina.cn
Received Date: 15 August 2022
Revised Date: 27 September 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.82074548), the Science and Technology Development Planning Project of Jilin Province, China (No.20220623026TC) and the Health Technology Innovation Project of Jilin Province, China (No.2021JC075).

A method was developed for screening protein binders from complicated ginseng samples by a streptavidin-coated 96-well plate format coupled with ultra-high performance liquid chromatography-orbitrap mass spectrometry (UHPLC-Orbitrap-MS). The Ricinus communis agglutinin 120 (RCA 120) coated 96-well plate and lysozyme coated 96-well plate (as control) were incubated with oligosaccharide standards respectively, and the compounds with the decreased peak areas in experimental group compared to those in the control group were detected as binders by UHPLC-ESI-MS. The factors such as incubation time, incubation temperature, and buffer, which might affect the binding affinity and reproducibility, were optimized. The potential of the approach was examined using the extracts of white ginseng, American ginseng and red ginseng. Three disaccharides and two trisaccharides were screened out from the extracts of white ginseng. As for the extracts of American ginseng, two disaccharides and two trisaccharides were observed to bind to RCA120. While no binders were detected in the extracts of red ginseng. Significant differences were observed in the relative binding degrees (RBDs) of the detected oligosaccharides in white ginseng and American ginseng binding to RCA 120. To our knowledge, it's the first time to reveal the differences and analogies in RCA 120-binding capabilities of oligosaccharides between the extracts of white ginseng and American ginseng, indicating the efficiency of the developed method for analysis of complicated samples.
1. [1]
2. [2]
  CHUNG I M, KIM J W, SEGUIN P, JUN Y M, KIM S H. Food Chem., 2012, 130(1):73-83.
3. [3]
  LUO D, FANG B. Carbohydr. Polym., 2008, 72(3):376-381.
4. [4]
  QI L W, WANG H Y, ZHANG H, WANG C Z, LI P, YUAN C S. J. Chromatogr. A, 2012, 1230:93-99.
5. [5]
  QIU Y, LU X, PANG T, MA C, LI X, XU G. J. Sep. Sci., 2008, 31(19):3451-3457.
6. [6]
  JIAO L, ZHANG X, LI B, LIU Z, WANG M, LIU S. Int. J. Biol. Macromol., 2014, 65:229-233.
7. [7]
  XU T, SHEN X, YU H, SUN L, LIN W, ZHANG C. J. Ginseng Res., 2016, 40(3):211-219.
8. [8]
  VARKI A. Glycobiology, 1993, 3(2):97-130.
9. [9]
  DAI Z, KAWDE A N, XIANG Y, LA BELLE J T, GERLACH J, BHAVANANDAN V P, JOSHI L, WANG J. J. Am. Chem. Soc., 2006, 128(31):10018-10019.
10. [10]
  LU W, PIETERS R J. Expert Opin. Drug Discov., 2019, 14(4):387-395.
11. [11]
  MIURA R, ETHELL I M, YAMAGUCHI Y. J. Neurochem., 2001, 76(2):413-424.
12. [12]
  COHEN M. Biomolecules, 2015, 5(3):2056-2072.
13. [13]
  LINMAN M J, TAYLOR J D, YU H, CHEN X, CHENG Q. Anal. Chem., 2008, 80(11):4007-4013.
14. [14]
  NAKAJIMA K, ODA Y, KINOSHITA M, KAKEHI K. J. Proteome Res., 2003, 2(1):81-88.
15. [15]
  BELLAPADRONA G, TESLER A B, GRVNSTEIN D, HOSSAIN L H, KIKKERI R, SEEBERGER P H, VASKEVICH A, RUBINSTEIN I. Anal. Chem., 2012, 84(1):232-240.
16. [16]
  LIANG P H, WANG S K, WONG C H. J. Am. Chem. Soc., 2007, 129(36):11177-11184.
17. [17]
  LIANG A, DESAI U R. Methods Mol. Biol., 2015, 1229:355-375.
18. [18]
19. [19]
  KITOVA E N, EL-HAWIET A, KLASSEN J S. J. Am. Soc. Mass Spectrom., 2014, 25(11):1908-1916.
20. [20]
  EL-HAWIET A, CHEN Y, SHAMS-UD-DOHA K, KITOVA E N, KITOV P I, BODE L, HAGE N, FALCONE F H, KLASSEN J S. Analyst, 2018, 143(2):536-548.
21. [21]
  YANG H, YAO W, WANG Y, SHI L, SU R, WAN D B, XU N, LIAN W, CHEN C, LIU S. Analyst, 2017, 142(4):670-675.
22. [22]
  JEONG D, LEE W Y. J. Electroanal. Chem., 2021, 903:115846.
23. [23]
  NARASIMHAN S, FREED J C, SCHACHTER H. Carbohydr. Res., 1986, 149(1):65-83.
24. [24]
  YANG Y, CUI X K, ZHONG M, LI Z J. Carbohydr. Res., 2012, 361:189-194.
25. [25]
  NORBERG O, DENG L, AASTRUP T, YAN M, RAMSTRÖM O. Anal. Chem., 2011, 83(3):1000-1007.
26. [26]
  ZHOU S, XU J, KONG M, YIP K M, XU J, SHEN H, ZHAO Z, LI S, CHEN H. J. Pharm. Biomed. Anal., 2017, 145:59-70.
1. [1]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
2. [2]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
3. [3]
  Yuxin CHEN , Yanni LING , Yuqing YAO , Keyi WANG , Linna LI , Xin ZHANG , Qin WANG , Hongdao LI , Wenmin WANG . Construction, structures, and interaction with DNA of two Sm^Ⅲ₄ complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1141-1150. doi: 10.11862/CJIC.20240258
4. [4]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
5. [5]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
6. [6]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
7. [7]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
8. [8]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
9. [9]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
10. [10]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
11. [11]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
12. [12]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
13. [13]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
14. [14]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
15. [15]
  Shijie Li , Ke Rong , Xiaoqin Wang , Chuqi Shen , Fang Yang , Qinghong Zhang . Design of Carbon Quantum Dots/CdS/Ta₃N₅ S-Scheme Heterojunction Nanofibers for Efficient Photocatalytic Antibiotic Removal. Acta Physico-Chimica Sinica, 2024, 40(12): 2403005-. doi: 10.3866/PKU.WHXB202403005
16. [16]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
17. [17]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
18. [18]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
19. [19]
  Yiling Wu , Peiyao Jin , Shenyue Tian , Ji Zhang . The Star of Sugar Substitutes: An Interview of Erythritol. University Chemistry, 2024, 39(9): 22-27. doi: 10.12461/PKU.DXHX202404034
20. [20]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(865)
HTML views(85)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142