Citation: YAN Lu, LI Xiao-dan, XIAO Ming-shu, PEI Hao. DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 516-524. doi: 10.19756/j.issn.0253-3820.221101 shu

DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate

YAN Lu ^1,2 ,
LI Xiao-dan ^1,2 ,
XIAO Ming-shu ^1,2 ,
PEI Hao ^1,2,,

1.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;2 Institute of Eco-Chongming, Shanghai 202162, China

Corresponding author: PEI Hao, peihao@chem.ecnu.edu.cn
Received Date: 25 February 2022
Revised Date: 17 March 2022

Fund Project: the China Postdoctoral Science Foundation(Nos. 2020TQ0097, 2021M701212)Supported by the National Natural Science Foundation of China(Nos. 22074041, 22104038)

Extracellular adenosine triphosphate(ATP) as a signaling molecule participates in a series of physiological processes, and its abnormal metabolism is closely related to many diseases(e.g., inflammation, infectious diseases). Developing methods for in situ detection of extracellular ATP is of great importance to elucidating the mechanism of relevant diseases and their diagnosis. This work reports DNA molecular machine-integrated microneedle patches. The developed microneedle patch allows for rapidly swelling in aqueous solution and in-situ sampling of target ATP molecules; the DNA molecular machine driven by ATP can realize fluorescence signal amplification. Benefiting from these traits, the microneedle patch-based analytical system shows excellent performance for ATP detection, including wide detection range(1-500 μmol/L), low limit of detection(1 μmol/L) and good selectivity.The result demonstrated that this microneedle patch-based analytical system enables in situ monitoring of ATP secreted by living cells under the stimulation of 0.1 mol/L K⁺, and the ATP concentration is determined to be(10.9 ± 1.6) μmol/L, consistent with previous work. This work provides a promising analytical platform for in situ monitoring of extracellular ATP.
- Detection of adenosine triphosphate,
- In situ detection,
- Microneedle patch,
- DNA molecular machine
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