Citation: CUI Ru-wen, FENG Tao-tao, GAO Nan, ZHANG Mei-ning. Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 859-868. doi: 10.19756/j.issn.0253-3820.221091 shu

Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor

Department of Chemistry, Renmin University of China, Beijing 100872, China

Corresponding author: ZHANG Mei-ning, mnzhang@ruc.edu.cn
Received Date: 22 February 2022
Revised Date: 25 March 2022

Fund Project: Supported by the National Natural Science Foundation of China(Nos. 21874152, 22174162).

Conducting polymers-based hydrogels, especially poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), have received increasing attention in the fields of bioelectronics and biosensors. However, current preparation methods of PEDOT:PSS hydrogel mostly use non-conductive hydrogel as a pre-template or severe treatment condition, which greatly limits the electrochemical biosensing application of hydrogels. Herein, we report a novel method for preparing PEDOT:PSS hydrogel. The PEDOT:PSS aqueous solution containing ethylene glycol is treated by vacuum drying,then the stable PEDOT:PSS hydrogel with three-dimensional cross-linking network is obtained by swelling. The prepared PEDOT:PSS hydrogel with micro/nano hierarchical pores and three-dimensional conductive network structure, has high conductivity(3321.33 ±129.41 S/m) and high stability in 2 months.The hydrogel-modified electrode not only has good property of heterogeneous electron transfer and mass transfer ability, but also can effectively resist non-specific adsorption of protein more than 1 month.The PEDOT:PSS hydrogel can stably entrap enzyme and maintain its activity, and the initial signal of the enzyme can be maintained more than 90% after 1 month. Therefore, the hydrogel provides a new strategy for constructing electrochemical sensors to detect interested substances in complex biological samples.
- Poly(3,
- 4-ethylenedioxythiophene):poly(styrene sulfonate),
- Conducting polymer hydrogel,
- Vacuum drying,
- Electrochemical biosensor,
- Anti-protein adsorption
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