Citation: MA Chong-bo, ZHOU Ming. Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 535-544. doi: 10.19756/j.issn.0253-3820.221067 shu

Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose

MA Chong-bo ,
ZHOU Ming ^,

Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education, National & Local United Engineering Laboratory for Power Batteries, Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Analysis and Testing Center, Department of Chemistry, Northeast Normal University, Changchun 130024, China

Corresponding author: ZHOU Ming, zhoum@nenu.edu.cn
Received Date: 10 February 2022
Revised Date: 14 March 2022

Fund Project: the “111” Project of China(No. B18012)the Jilin Provincial Department of Human Resources and Social Security,the Jilin Provincial Department of Education(China),the Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province(China),Analysis and Testing Center of Northeast Normal University(China)the Natural Science Foundation of Jilin Province,China(No. 212558JC010484610)Supported by the National Natural Science Foundation of China(No. 22004014)the Fundamental Research Funds for the Central Universities of China (Nos. 2412020QD007, JGPY201802, 2412020ZD006, 2412019QD008)

A method for synthesis of an amphiphilic aerogel with remarkable peroxidase-like activity was developed by using poly(vinyl alcohol) (PVA) as skeleton and maleic acid(MA) as auxiliary crosslinker.Large amount of carboxyl and ester groups could be exposed on aerogel surface by tuning the dosage of MA, which acted as the active sites and binding sites for the substrate material. A colorimetric method for glucose sensing by the catalyzed chromogenic reaction between 3, 3', 5, 5'-tetramethylbenzidine and H₂O₂, was thus established. The linear detection range of glucose in buffer system was 17.4-80.0 μmol/L, with limit of detection(3σ) of 17.4 μmol/L. While in diluted human serum, the linear detection range was 27.4 μmol/L-1.0 mmol/L, with limit of detection(3σ) of 27.4 μmol/L. This method was used in detection of glucose in human serum, with recoveries of 96.8%-103.0% and relative standard deviations of 0.8%-3.9%, and the detection results were highly consistent with those obtained from the commercial glucose meter. This study innovatively developed a three-dimensional macroscope organic aerogel as a new type of artificial enzyme, broadening the design philosophy and enriching the synthesis strategy. It was promising in construction of portable sensors by virtue of the lightness in weight and eligible mechanical properties.
- Amphiphilic aerogel,
- Artificial enzyme,
- Peroxidase,
- Glucose sensing,
- Portable sensor
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