Citation: MA Ru-long, HAO Qian, LIANG Meng-jia, YU Yan-kai, NIU Na, CHEN Li-gang. Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 545-553. doi: 10.19756/j.issn.0253-3820.221035 shu

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China

Corresponding author: NIU Na, CHEN Li-gang,
Received Date: 19 January 2022
Revised Date: 28 February 2022

Fund Project: the Natural Science Foundation of Heilongjiang Province,China(No. B2008001)Supported by the National Natural Science Foundation of China(No. 21401019)the Provincial College Students’ Innovation Training Program of Northeast Forestry University(No. 202110225341)

The MoS₂-modified straw carbon dots nanozyme(Mo, S-CDs) was prepared by hydrothermal synthesis for detection of content of uric acid in human urine. This work fully exploited the inherent advantages of straw structure and realized the recycling of biomass waste. The characterization of Mo, S-CDs showed that Mo and S elements were successfully modified into the structure of straw carbon dots, and the Mo, S-CDs were spherical with abundant functional groups on the surface to ensure their good dispersion in aqueous medium and amorphous carbon structure at crystal level. The Mo, S-CDs had the activity of mimetic peroxidase and showed high catalytic efficiency. They were able to rapidly catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine in a system containing H₂O₂ to generate chromogenic product, which visually showed a characteristic blue color signal in response and reached a peak absorbance at 652 nm.The cascade catalytic sensing system of uric acid-Mo, S-CDs was constructed based on the sensing ability of Mo, S-CDs for H₂O₂ generation catalyzed by uric acid, which was applied to the detection of uric acid in human urine. The sensing system had good linear range(5-100 μmol/L), low detection limit(1.8 μmol/L), satisfied recovery(96.8%-106.1%) and good relative standard deviation(less than 5%) in detection of uric acid. The results showed that Mo, S-CDs had advantages over natural peroxidases in terms of wide applicable temperature range and high stability, which would make them more widely useful in fields such as life analysis.
- Carbon dots nanozyme,
- Straw,
- Peroxidase,
- Uric acid,
- Urine
1. [1]
  AGNOLETTI D, CICERO A F G, BORGHI C. Cardiol. Clin., 2021, 39(3):365-376.
2. [2]
  HAN M, KIM H, KIM H J, KANG E, KIM Y S, CHOIK H, KIM S W, AHN C, OH K H. BMC Nephrol., 2021,22(1):247.
3. [3]
4. [4]
  JEN J F, HSIAO S L, LIU K H. Talanta, 2002, 58(4):711-717.
5. [5]
6. [6]
7. [7]
8. [8]
  ZHAO J J, ZHAO L M, LAN C Q, ZHAO S L. Sens. Actuators, B, 2016, 223:246-251.
9. [9]
  WU C Y, ZHU L J, LU Q J, LI H T, ZHANG Y Y, YAO S Z. Microchim. Acta, 2019, 186(12):754-761.
10. [10]
  BADOEI-DALFARD A, SOHRABI N, KARAMI Z, SARGAZI G. Biosens. Bioelectron., 2019, 141:111420.
11. [11]
12. [12]
  SUN Y, CHEN J, QI H, SHI Y. J. Chromatogr. B, 2015, 1004:53-59.
13. [13]
  LIU H, GU C, HOU C, YIN Z, FAN K, ZHANG M. Sens. Actuators, B, 2016, 224:857-862.
14. [14]
  FANG A, WU Q, LU Q, CHEN H, LI H, LIU M, ZHANG Y, YAO S. Biosens. Bioelectron., 2016, 86:664-670.
15. [15]
  PAN Y D, YANG Y F, PANG Y J, SHI Y, LONG Y J, ZHENG H Z. Talanta, 2018, 185:433-438.
16. [16]
  SUN H J, ZHOU Y, REN J S, QU X G. Angew. Chem., Int. Ed., 2018, 57(30):9224-9237.
17. [17]
  GAO L Z, ZHUANG J, NIE L, ZHANG J B, ZHANG Y, GU N, WANG T H, FENG J, YANG D L, PERRETT S,YAN X Y. Nat. Nanotechnol., 2007, 2(9):577-583.
18. [18]
  DING H, HU B, ZHANG B, ZHANG H, YAN X Y, NIE G H, LIANG M M. Nano Res., 2021, 14:570-583.
19. [19]
20. [20]
21. [21]
  DEVI P, SAINI S, KIM K H. Biosens. Bioelectron., 2019, 141:111158.
22. [22]
  TAN J Y, LI Y, TAN X, WU H G, LI H, YANG S. Front. Chem., 2021, 9:696030.
23. [23]
  LI F, HE T S, WU S H, PENG Z J, QIU P, TANG X M. Microchem. J., 2021, 164:105987.
24. [24]
  DING H, WEI J S, ZHANG P, ZHOU Z Y, GAO Q Y, XIONG H M. Small, 2018, 14(22):1800612.
25. [25]
  WANG X J, WU Q, JIANG K L, WANG C X, ZHANG C. Sens. Actuators, B, 2017, 252:183-190.
26. [26]
  BANO D, KUMAR V, SINGH V K, HASAN S H. New J. Chem., 2018, 42(8):5814-5821.
27. [27]
  ZHAO Y H, HUANG Y, WU J L, ZHAN X I, XIE Y Y, TANG D Y, CAO H Y, YUN Y. RSC Adv., 2018, 8(13):7252-7259.
28. [28]
  JIANG B, DUAN D M, GAO L Z, ZHOU M J, FAN K L, TANG Y, XI J Q, BI Y H, TONG Z, GAO G F, XIE N,TANG A F, NIE G H, LIANG M M, YAN X Y. Nat. Protoc., 2018, 13(7):1506-1520.
29. [29]
  HUANG Z, HE W T, SHEN H, HAN G J, WANG H, SU P, SONG J Y, YANG Y. Talanta, 2021, 230:122337.
30. [30]
  CHEN Q, LIU M L, ZHAO J N, PENG X, CHEN X J, MI N X, YIN B D, LI H T, ZHANG Y Y, YAO S Z. Chem.Commun., 2014, 50:6771-6774.
31. [31]
  LIN L P, SONG X H, CHEN Y Y, RONG M C, ZHAO T T, WANG Y R, JIANG Y Q, CHEN X. Anal. Chim. Acta,2015, 869:89-95.
32. [32]
  DING C P, YAN Y H, XIANG D S, ZHANG C L, XIAN Y Z. Microchim. Acta, 2016, 183:625-631.
33. [33]
  JIN L H, MENG Z, ZHANG Y Q, CAI S J, ZHANG Z H, LI C, SHANG L, SHEN Y H. ACS Appl. Mater.Interfaces, 2017, 9(11):10027–10033.
34. [34]
  NIRALA N R, VINITA, PRAKASH R. Microchim. Acta, 2018, 185(4):224.
35. [35]
  SUN H J, ZHAO A D, GAO N, LI K, REN J S, QU X G. Angew. Chem., Int. Ed., 2015, 54(24):7176-7180.
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Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine