Citation: LI Jun-Feng, ZHANG Qing-Rong, FU Yan-Feng, LI Si-Ying, LIU Yu-Lin, ZHOU Jing, CAI Ming-Jun, YANG Guo-Cheng, SHAN Yu-Ping. Study on Cytotoxicity of Nanodrug Carrier-Polyamidoamine Dendrimers[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(6): 999-1007. doi: 10.19756/j.issn.0253-3820.211129 shu

Study on Cytotoxicity of Nanodrug Carrier-Polyamidoamine Dendrimers

1.
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: SHAN Yu-Ping, shanyp@mail.ccut.edu.cn
Received Date: 19 February 2021
Revised Date: 14 April 2021

Fund Project: Supported by the National Natural Science Foundation of China(No.201773017).

Due to the excellent drug loading capacity, the amphiphobic dendritic macromolecule polyamide amine (PAMAM) has become an important drug carrier in nanodrug transport system. Therefore, real-time measurement of the cytotoxicity of different generations and different terminal groups of PAMAM in the near physiological environment is particularly important for drug carrier screening. In this study, taking the advantages of atomic force microscopy (AFM)-based nanoindentation technology for measuring the mechanical properties of individual cells under physiological conditions, the cell mechanical properties (Young's modulus) were compared after coincubation with different generations and different terminal groups of PAMAM at different concentrations for different tme, and the cytotoxicity of PAMAM was analyzed. Because there was no obvious charge effect, the change of Young's modulus of HeLa cells coincubated with G2-PAMAM-NH₂ and G5-PAMAM-OH at different concentrations for different time was not obvious. The Young's modulus of HeLa cells was 0.61, 0.45, 0.46 and 0.22 kPa after coincubation with 0.5 μmol/L G5-PAMAM-NH₂ for 0 h, 0.5 h, 1 h and 2 h, respectively. The Young's modulus of HeLa cells were 0.30, 0.20, 0.17 and 0.22 kPa after coincubation with G5-PAMAM-NH₂ for 2 h at 0.5, 1.0, 2.0 and 5.0 μmol/L, respectively. It was found that the coincubation time was the dominant factor of cytotoxicity, while the cytotoxicity of G5-PAMAM-COOH was concentration-dependent. This study would lay a theoretical foundation for the better application of PAMAM in nanomedicine as a nanodrug carrier.
- Atomic force microscopy,
- Nanoindentation,
- Polyamidoamine,
- Cytotoxicity,
- Cell mechanics
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