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Citation: ZHU Li-Li, CHEN Da-Yong, TONG Wen-Jun. Oxidation Monitoring of Cyclolinopeptide in Flaxseed by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 512-519. doi: 10.19756/j.issn.0253-3820.211119 shu

Oxidation Monitoring of Cyclolinopeptide in Flaxseed by High Performance Liquid Chromatography-Tandem Mass Spectrometry

School of Chemistry and Materials, Nanjing Normal University, Nanjing 210023, China

Corresponding author: TONG Wen-Jun, wjtong@njnu.edu.cn
Received Date: 11 February 2021
Revised Date: 3 March 2021

Orbitrap based high performance liquid chromatography-high resolution tandem mass spectrometry (HPLC-HRMS) was used to study the post-translational modification (PTM) of cyclopeptides in commercial flaxseed extract, flaxseed varieties and processing conditions. The oxidation degree and oxidation sites of cyclolinopeptides were used to evaluate the quality of flaxseed oil under different processing conditions. The identification and quantitative methods were established after optimizing analytical conditions. By exact mass and tandem mass spectrometry analysis, 16 and 21 oxidized cyclolinopeptides were identified in raw and cooked flaxseed extracts, respectively. The oxidation degree was presented as the ratio of the peak area of cyclolinopeptides with and without oxidation. The results showed that the high temperature drying process had a significant effect on the oxidation of methionine residues in cyclolinopeptides and the oxidation level of brown flaxseed was more sensitive to temperature and oxygen conditions than golden one.
- Flaxseed,
- Cyclolinopeptide,
- Oxidation degree,
- High performance liquid chromatography,
- High resolution tandem mass spectrometry
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