Citation: LIN Chu-Hui, CHAI Rui-Ping, ZHANG Lu, FU Zhi-Bo, ZHANG Hong-Yang, ZHANG Min, HU Ping. Determination of Six Kinds of Bile Acids in NiuhuangJiedu Tablets Using High Performance Liquid Chromatography Coupled with Charged Aerosol Detector[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(5): 764-771. doi: 10.19756/j.issn.0253-3820.210910 shu

Determination of Six Kinds of Bile Acids in NiuhuangJiedu Tablets Using High Performance Liquid Chromatography Coupled with Charged Aerosol Detector

1.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;
2.
Thermo Fisher Scientific China Co. Ltd., Shanghai 200000, China;
3.
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: HU Ping, huping@ecust.edu.cn
Received Date: 31 December 2021
Revised Date: 27 February 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.81973285).

A high performance liquid chromatography-charge aerosol detector (HPLC-CAD) method for determination of six kinds of bile acids(Glycocholicacid, hyodeoxycholic acid, cholic acid, glycodeoxycholic acid, chenodeoxycholic acid and deoxycholic acid) in Niuhuang Jiedu tablets was developed. The tablet samples were extracted by ultrasonication in 70% methanol and concentrated under nitrogen flow. The separation of bile acids was achieved on an Agilent Poroshell 120 EC C₁₈ column (4.6 mm × 100 mm, 2.7 μm) with gradient elution using methanol and aqueous solution containing 0.1% formic acid and 5 mmol/L ammonium formate. CAD evaporation temperature was set at 55℃ and the power function value (PFV) was 1.35. The linearity ranges were 2.10-972 μg/mL for glycocholic acid, 2.09-1046 μg/mL for hyodeoxycholic acid, 2.05-978 μg/mL for cholic acid, 2.56-1022 μg/mL for glycodeoxycholic acid, 1.25-658 μg/mL for chenodeoxycholic acid and 2.05-1025 μg/mL for deoxycholic acid, respectively. All relative coefficients (R²) were higher than 0.9991. The limits of detection and the limits of quantitation ranged from 0.86 to 1.53 μg/mL and 1.20 to 2.56 μg/mL, respectively. The average recoveries of six kinds of bile acids were in the range of 94.2%-105.3% with RSD ≤ 1.63%. The method was used to determine the real tablet samples, and the results indicated that the contents of six bile acids had an obvious difference among the Niuhuang Jiedu tablets of different manufacturers. The HPLC-CAD method for the determination of six kinds of bile acids was sensitive, accurate and reliable, which could be applied to evaluate the quality of the Niuhuang Jiedu tablets.
- Niuhuang Jiedu tablet,
- High performance liquid chromatography,
- Charged aerosol detector,
- Bile acid
1. [1]
2. [2]
  HU Z, HE L C, ZHANG J, LUO G A.J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2006, 837(1-2):11-17.
3. [3]
4. [4]
  CHARLES T, ROBERTO P, MARK P, JOHAN A, KRISTINA S. Nat. Rev. Drug Discovery, 2008, 7(8):678-693.
5. [5]
  YANG F, HE Y Q, LIU H X, TSUEI J, JIANG X Y, YANG L, WANG Z T, WAN Y J Y. Biochem. Pharmacol., 2014, 91(4):483-489.
6. [6]
  PERINO A, POLS T W H, NOMURA M, STEIN S, PELLICCIARI R, SCHOONJANS K. J. Clin. Invest., 2014, 124(12):5424-36.
7. [7]
  REITER S, DUNKEL A, DAWID C, HOFMANN T. J. Agric. Food Chem., 2021, 69(36):10572-10580.
8. [8]
  ASHLEY C J, OMAR G Q, VALERIE S F, PHILIPPE Z, CARMELO Q, LUIGI B, ANNE T, JULIE C, DANIELA F, MARCUS H, CATHERINE P, VINCENT S, CAMILLE A, SANDRINE Q, VALENTINE G, NATHALIE H, ALESSIA P, ALEXIA D, MARLENE M, THIERRY L L, SAMANTHA C, NATHALIE D, ASTRID C, DELPHINE G, BENOIT D, GILLES M, DAVID D, FREDRIK B, VINCENT P, GIOVANNI M, BART S, KRISTINA S, DANIELA C. Cell Metab., 2021, 33(7):1483-1492.
9. [9]
  BETHAN L C, LESLIE R S, KEVIN J W, PAULINE M, ANGELA C, KELSEY E J, ALVIVN P C, MADELAINE C B S, ANNIKA W, JULIANNE W A, WILLIAM B, JAMES W, ANNA C C, LIU Y Y, ANDERS T, PETER J M, BRIAN G D, JULIA J M, HANNS U M, ELIZABETH J T, PETER A E, THOMAS Q A V. Cell Metab., 2021, 33(8):1671-1684.
10. [10]
  LUO M M, YAN J B, WU L Y, WU J T, CHEN Z, JIANG J P, CHEN Z Y, HE B H. J. Immunol. Res., 2021, 2021:2264737.
11. [11]
  WU Y M, WANG X C, WU Q Z, WU X P, LIN X C, XIE Z H. Anal. Methods, 2010, 2(12):1927-1933.
12. [12]
  SHI Y, XIONG J, SUN D M, LIU W, WEI F, MA S C, LIN R C. J. Sep. Sci., 2015, 38(16):2753-2762.
13. [13]
  KONG W J, JIN C, LIU W, XIAO X H, ZHAO Y L, LI Z L, ZHANG P, LI X F. Food Chem., 2010, 120(4):1193-1200.
14. [14]
  XIONG J, ZHENG T J, SHI Y, WEI F, MA S C, HE L, WANG S C, LIU X S. J. Pharm. Biomed. Anal., 2019, 174:50-56.
15. [15]
  CHEN M J, LIU C, SHEN Y M, ZOU J F, ZHANG Z M, WAN Y, YANG L, JIANG S, QIAN D W, DUAN J N. J. Chromatogr. Sci., 2021, 59(9):871-876.
16. [16]
17. [17]
  KAKIYAMA G, MUTO A, TAKEI H, NITTONO H, MURAI T, KUROSA W A T, HOFMANNA F, PANDAK W M, BAJAJJS J. Lipid Res., 2014, 55(5):978-990.
18. [18]
  ZHENG J P, YE C, HU B F, YANG H B, YAO Q F, MA J, LIU Y, LIU H T. Biotechnol. Appl. Biochem., 2020, 68(6):1332-1341.
19. [19]
  LIU W X, CHENG X L, GUO X H, HU X R, WEI F, MA S C. Pharm. Biomed. Anal., 2020, 179:1-8.
20. [20]
  SHAFAEI A, REES J, CHRISTOPHERSEN C T, DEVINE A, BROADHURST D, BOYCE M C. Anal. Chim. Acta, 2021, 1150:1-9.
21. [21]
  YANG M T, TAN D P, LU A J, QIN L, WANG C H, LING H, LU Y L, HE Y Q. Int. J. Anal. Chem., 2021, 2021:5209618.
22. [22]
  WU L L, ZHANG S N, ZHOU L H, XIONG H S, GONG X C, ZHANG S J, PAN J Y, QU H B. Phytochem. Anal., 2021, 32(6):942-956.
23. [23]
  LIU A X, XU T T, YANG W N, ZHOU D D, SHA Y W. J. Anal. Methods Chem., 2021, 2021:6616854.
24. [24]
  XIE M J, YU Y T, ZHU Z Y, DENG L P, REN B, ZHANG M. J. Pharm. Biomed. Anal., 2021, 201:114087.
25. [25]
  XU X Y, WANG S M, WANG H M, HU W D, HAN L F, CHEN B X, LI X, WANG H D, LI H F, GAO X M, GUO D, YANG W Z. J. Chromatogr. A, 2021, 1655:462504.
26. [26]
  XIE Q, TIAN H M, HUAN X H, CAO L L, WANG Y, CHENG X M, NING C G, HU F D, WANG C H. Phytochem. Anal., 2022, 33:262-271.
27. [27]
  NGUYEN H T, VU-HUYNH K L, NGUYEN H M, LE H T, LE T H V, PARK J L, NGUYEN M D. Molecules, 2021, 26(17):5373.
28. [28]
  WANG C, CHAO I C, QIN Y, ZHANG W X, ZHAO J, ZHANG Q W. J. Pharm. Biomed. Anal., 2022, 210:114545.
29. [29]
  VERTZONI M, ARCHONTAKI H, REPPAS C. J. Lipid Res., 2008, 49(12):2690-2695.
30. [30]
31. [31]
  PAWELLEK R, MUELLNER T, GAMACHE P, HOLZGRABE U. J.Chromatogr. A, 2021, 1637:461844.
32. [32]
  GAMACHE P H. Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques. New Jersey:John Wiley & Sons, 2017.
33. [33]
  AHMAD I A H, BLASKO A, WANG H, LU T, MANGION I, REGALADO E L. J. Chromatogr. A, 2021, 1641:461997.
34. [34]
1. [1]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
2. [2]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
3. [3]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
4. [4]
  Gengjia Chen , Junjie Ou . Application of the van Deemter Equation in Instrumental Analysis Teaching: A Case of Organic Polymer Monolithic Columns. University Chemistry, 2025, 40(11): 362-368. doi: 10.12461/PKU.DXHX202502003
5. [5]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
6. [6]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
7. [7]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
8. [8]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . Catalytic Effect of H₃PW₁₂O₄₀ on Hydrogen Storage of MgH₂. Acta Physico-Chimica Sinica, 2025, 41(1): 100001-0. doi: 10.3866/PKU.WHXB202308032
9. [9]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
10. [10]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
11. [11]
  Xudong Liu , Huili Fan , Junping Xiao , Min Yang , Yan Li . Teaching Approaches to the A_E + A_N Mechanism of Electrophilic Addition Reactions between Olefins and Inorganic Acids in Organic Chemistry. University Chemistry, 2025, 40(7): 367-372. doi: 10.12461/PKU.DXHX202409041
12. [12]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
13. [13]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
14. [14]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
15. [15]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
16. [16]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
17. [17]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
18. [18]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
19. [19]
  Binbin Liu , Yang Chen , Tianci Jia , Chen Chen , Zhanghao Wu , Yuhui Liu , Yuhang Zhai , Tianshu Ma , Changlei Wang . Hydroxyl-functionalized molecular engineering mitigates 2D phase barriers for efficient wide-bandgap and all-perovskite tandem solar cells. Acta Physico-Chimica Sinica, 2026, 42(1): 100128-0. doi: 10.1016/j.actphy.2025.100128
20. [20]
  Jiayao Wang , Guixu Pan , Ning Wang , Shihan Wang , Yaolin Zhu , Yunfeng Li . Preparation of donor-π-acceptor type graphitic carbon nitride photocatalytic systems via molecular level regulation for high-efficient H₂O₂ production. Acta Physico-Chimica Sinica, 2025, 41(12): 100168-0. doi: 10.1016/j.actphy.2025.100168

Get Citation

PDF

XML

Metrics

PDF Downloads(13)
Abstract views(989)
HTML views(189)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142