Citation: WANG Li-Ying, ZHANG Xin-Yu, LIU Jia-Ming, WU Xin-Rong, ZHANG Guang-Chao, YANG Ming-Shuo, ZHOU Yang, ZHANG Yan-Qi. Analysis of Molecular Mechanism of Myocardial Remodeling[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(5): 711-719. doi: 10.19756/j.issn.0253-3820.210856 shu

Analysis of Molecular Mechanism of Myocardial Remodeling

1.
Basic Medicine College of Beihua University, Jilin 132013, China;
2.
Chinese Institute of Standardization, Beijing 100191, China;
3.
College of Chemistry&Chemical Engineering, Lanzhou University, Lanzhou 730000, China

Corresponding author: ZHANG Yan-Qi, zhangyq@cnis.gov.cn
Received Date: 23 November 2021
Revised Date: 31 December 2021

Fund Project: Supported by the Science and Technology Research Project of the Department of Education of Jilin Province (No.JJKH20220067KJ), the Science and Technology Development Plan Project of Jilin Province (No.20190303038SF), the Science and Technology Development Project of Jilin City (No.20190302018) and the Jilin Provincial Health Technology Innovation Project (No.2019J051).

The roles of various signaling molecules in myocardial remodeling (MR) in vivo and in vitro were investigated and the mechanisms based on the combination of various detection techniques was explored in this study. First, the in vivo MR model of Wistar rat was established using isoprenaline (Iso). AngiotensinⅡ (AngⅡ) induced proliferation of neonatal rat myofibroblasts (myoFbs) and thus set up the MR model in vitro. Further, Western blot was applied to detection of the expression of PKCα (protein kinase Ca, PKCα) in vivo, while methyl thiazolyl tetrazolium (MTT) assay was adopted for detection of myoFbs proliferation in vitro. Iso increased both the content of hydroxyproline (Hyp) and the expression of PKCα in myocardial tissue significantly. At the same time, it was found that AngⅡ (10^-7 mol/L) promoted the proliferation of myoFbs significantly. Immunocytochemical staining and laser confocal microscopy were used to detect expression and content of p-PKCα, extracellular signal regulating protein kinase (ERKl/2) and reactive oxygen species (ROS) induced by AngⅡ in myoFbs. The results showed that the formation of MR was related to the increase of expression of ROS, p-PKCα and p-ERKl/2.
- Myocardial remodeling,
- Myofibrosis,
- Myofibroblasts,
- Protein kinase Cα,
- Reactive oxygen species
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沈阳化工大学材料科学与工程学院沈阳 110142