Citation: FANG Li, QIU Feng-mei. Fast Determination of Bongkrekic Acid and Iso-bongkrekic Acid in Human Plasma and Urine by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotope Dilution[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 634-642. doi: 10.19756/j.issn.0253-3820.210851 shu

Fast Determination of Bongkrekic Acid and Iso-bongkrekic Acid in Human Plasma and Urine by Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotope Dilution

FANG Li ^1,, ,
QIU Feng-mei ²

1.
Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Municipal District Center for Disease Control and Prevention, Zhoushan 316021, China;2 Putuo District Center for Disease Control and Prevention, Zhoushan 316100, China

Corresponding author: FANG Li, fangli123@126.com
Received Date: 19 November 2021
Revised Date: 18 March 2022

Fund Project: the Medical and Health Science and Technology Program of Zhoushan(No.2020YA01)Supported by the Science and Technology Program of Zhoushan(No.2021C31071)

A method for fast quantitative analysis of bongkrekic acid and iso-bongkrekic acid in human plasma and urine by liquid chromatography-tandem mass spectrometry(LC-MS/MS) coupled with isotope dilution was developed. The sample was extracted with acetonitrile, separated on a C₁₈ column(100 mm × 2.1 mm, 1.9 μm), and detected in selected reaction monitoring(SRM) mode via positive electrospray ionization. The internal standard method was used for quantitation with solvent standard curve. Bongkrekic acid and iso-bongkrekic acid showed good linearity in the concentration range of 2.0-400.0 μg/L with correlation coefficients of higher than 0.9984. The quantitation limits of bongkrekic acid and iso-bongkrekic acid in human plasma and urine were 2.0 μg/L. The intra-and inter-day recoveries were 89.7%-109.6% and 86.2%-104.2%, respectively, at spiked concentrations of 2.0 μg/L, 10.0 μg/L, 40.0 μg/L, and 200.0 μg/L. The inter-and intra-day relative standard deviations(RSDs) were lower than 9.8%. The method was accurate, fast and easy to operate, and could satisfy the requirements of public health emergency testing or clinical testing.
- Isotope dilution,
- Plasma,
- Urine,
- Lquid chromatography-tandem mass spectrometry,
- Bongkrekic acid
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