Citation: HU Shuang, HUA Zhen-Dong, HUANG Yu, CHENG Fang-Bin, LIU Yao. Fast Separation and Detection of Fentanyls Isomers by Ultra Performance Convergence Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 964-972. doi: 10.19756/j.issn.0253-3820.210839 shu

Fast Separation and Detection of Fentanyls Isomers by Ultra Performance Convergence Chromatography-Mass Spectrometry

HU Shuang ^1,2 ,
HUA Zhen-Dong ^3,, ,
HUANG Yu ⁴ ,
CHENG Fang-Bin ⁵ ,
LIU Yao ^1,,

1.
School of Criminal Investigation, People's Public Security University of China, Beijing 100038, China;
2.
Department of Criminal Science and Technology, Beijing Police College, Beijing 102202, China;
3.
Key Laboratory of Drug Monitoring and Control, Anti-Drug Information Technology Center of the Ministry of Public Security, Beijing 100193, China;
4.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China;
5.
Zhongshan Public Security Bureau Criminal Detachment, Zhongshan 528403, China

Corresponding author: HUA Zhen-Dong, LIU Yao,
Received Date: 16 November 2021
Revised Date: 5 March 2022

Fund Project: Supported by the Scientific Research Project of Beijing Municipal Education Commission(No. KM202114019001) and the Science and Technology to Strengthen Basic Work Project of Ministry of Public Security(No. 2020GABJC21).

As a new class of psychoactive substances, fentanyls have the characteristics of wide variety,rapid replacement and extremely similar structures. The accurate identification of their isomers is a major problem faced by drug analysts. Ultra performance convergence chromatography is a powerful tool to achieve efficient separation of isomers. Therefore, a new method was established for fast separation and detection of fentanyls by ultra performance convergence chromatography-mass spectrometry in this work. The separation conditions of the chromatographic column, modifiers and column temperature were optimized. The separation and detection was conducted on Waters ACQUITY UPC² BEH column with supercritical fluid CO₂ as primary mobile phase, methanol containing 20 mmol/L ammonium formate as the modifier, column temperature at 50℃ and a Xevo TQD triple quadrupole as mass spectrometer. A total of 13 kinds of fentanyls were completely separated within 6.5 min with an above 1.5-resolution to all the isomers. The chromatographic peaks were perfectly symmetric. Methodological statistics showed that, 9 kinds of common drugs did not interfere with the determination of 13 kinds of target substances;the detection limits were 0.01-0.05 ng/mL and the relative standard deviations of the retention time were less than 0.6%. Compared with the conventional ultra-high performance liquid chromatography-mass spectrometry detection method, this method had significant advantages in the separation of isomers,which fully met the anti-drug public security departments' needs for the accurate identification of fentanyls.
- Ultra performance convergence chromatography,
- Mass spectrometry,
- Fentanyls,
- Isomers,
- New psychoactive substances
1. [1]
  VARDANYAN R S, HRUBY V J. Future Med. Chem., 2014, 6(4):385-412.
2. [2]
  MOUNTENEY J, GIRAUDON I, DENISSOV G, GRIFFITHS P. Int. J. Drug Policy, 2015, 26(7):626-631.
3. [3]
  SUZUKI S. Forensic Sci. Int., 1989, 43(1):15-19.
4. [4]
  RITTGEN J, PUTZ M, ZIMMERMANN R. Electrophoresis, 2012, 33(11):1595-1605.
5. [5]
  GILBERT N, ANTONIDES L H, SCHOFIELD C J, COSTELLO A, KILKELLY B, CAIN A R, DALZIEL P R V, HORNER K, MEWIS R E, SUTCLIFFE O B. Drug Test. Anal., 2020, 12(6):798-811.
6. [6]
  ROJKIEWICZ M, MAJCHRZAK M, CELINSKI R, KUS P, SAJEWICZ M. Drug Test. Anal., 2017, 9(3):405-414.
7. [7]
  KANAMORI T, IWATA Y T, SEGAWA H, YAMAMURO T, KUWAYAMA K, TSUJIKAWA K, INOUE H. J.Forensic Sci., 2017, 62(6):1472-1478.
8. [8]
  BUCHALTER S, MARGINEAN I, YOHANNAN J. J. Chromatogr. A, 2019, 1596:183-193.
9. [9]
  LURIE I S, IIO R. J. Chromatogr. A, 2009, 1216(9):1515-1519.
10. [10]
  BUSARDO F P, CARLIER J, GIORGETTI R, TAGLIABRACCI A, PACIFICI R, GOTTARDI M, PICHINI S.Front. Chem., 2019, 7(2):184-197.
11. [11]
  SALEMMILANI R, MOSKIVITS M, MEINHART C D. Analyst, 2019, 144(2):3080-3087.
12. [12]
  BERNAL J L, MARTIN M T, TORIBIO L. J. Chromatogr. A, 2013, 1313(SI):24-36.
13. [13]
14. [14]
15. [15]
16. [16]
  PAUK V, ZIHLOVA V, BOROVCOVA L, HAVLICEK V, SCHUG K, LEMR K. J. Chromatogr. A, 2015, 1423:169-176.
17. [17]
  BREITENBACH S, ROWE W F, MCCORD B. J. Chromatogr. A, 2016, 1440:201-211.
18. [18]
  CARNES S, BRIEN S, SZEWCZAK A, TREMEAU C L, ROWE W F, MCCORD B, LURIE I S. J. Sep. Sci., 2017, 40(17):3545-3556.
19. [19]
  SEGAWA H, KUSAKABE K, ISHII A, KATO N, IWATA Y T, YAMAMURO T, KANAMORI T. Anal. Sci. Adv., 2020, 1(1):22-33.
20. [20]
1. [1]
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2. [2]
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3. [3]
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16. [16]
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17. [17]
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18. [18]
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19. [19]
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20. [20]
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