Citation: ZHAO Dan, MA Xiao, LI Na, WANG Fang, CHEN Chuan-Xia, SUN Jian. Research Progress of Fluorescent Detection Methods for Alkaline Phosphatase Activity[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1804-1815. doi: 10.19756/j.issn.0253-3820.210654 shu

Research Progress of Fluorescent Detection Methods for Alkaline Phosphatase Activity

ZHAO Dan ¹ ,
MA Xiao ^1,, ,
LI Na ¹ ,
WANG Fang ¹ ,
CHEN Chuan-Xia ^2,, ,
SUN Jian ^3,,

1.
Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China;
2.
School of Materials Science and Engineering, University of Jinan, Jinan 250022, China;
3.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: MA Xiao, CHEN Chuan-Xia, SUN Jian,
Received Date: 28 July 2021
Revised Date: 12 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22104046, 21974132, 21904048), the Natural Science Foundation of Henan Province, China (No.202300410285), the Project of Science and Technology Department of Henan Province, China (Nos.212102210122, 212102310409), the Scientific Research Foundation of Luoyang Institute of Science and Technology (No.2017BZ16) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (No.2018258).

Alkaline phosphatase (ALP), which can catalyze the dephosphorylation of various phosphorylated species, is an essential enzyme in a variety of mammalian tissues. The exploration of novel approaches for detection of ALP with high selectivity and sensitivity and intracellular imaging in living organisms is of vital importance in human health, clinical diagnosis and environmental analysis. With the development of fluorescent probes, many advantages for ALP detection, such as simple operation, non-invasive, in-situ detection, real-time imaging and so on, have been gradually highlighted. The fluorescent methods are categorized by different response mechanisms for ALP activity sensing:difference in the fluorescence between the phosphorylated substrate and corresponding product; the interaction of certain metal ions and phosphorylated substrates; the in-situ fluorogenic reaction drived by the ALP-enabled dephosphorylation process; inner filter effect; the fluorescence energy transfer system and so on. In this review, we focused on the research progresses of the fluorescent methods in detection of ALP in recent years. Furthermore, the prospects of ALP in the near future were also discussed.
- Alkaline phosphatase,
- Fluorescence detection,
- Response mechanism,
- Phosphorylated species,
- Review
1. [1]
  MILLAN J L. Purinerg. Signal., 2006, 2(2):335-341.
2. [2]
  KHAN A R, AWAN F R, NAJAM S, ISLAM M, SIDDIQUE T, ZAIN M. J. Pak. Med. Assoc., 2015, 65(11):1182-1185.
3. [3]
  OOI K, SHIRAKI K, MORISTHITA Y. J. Clin. Lab. Anal., 2010, 21(3):133-139.
4. [4]
  DE LARICA R, STEVENS M M. Nat. Nanotechnol., 2012, 7(12):821-824.
5. [5]
  KIM E K, KEEM J O, YUN H S, JUNG J, CHUNG B H. Chem. Commun., 2015, 51(15):3270-3272.
6. [6]
  LIU Y H, GUO W L, SU B. Chin. Chem. Lett., 2019, 30(9):1593-1599.
7. [7]
  IQBAL J. Anal. Biochem., 2011, 414(2):226-231.
8. [8]
  RUAN C M, WANG W, GU B H. Anal. Chem., 2006, 78(10):3379-3384.
9. [9]
  ZHENG J, SONG D D, ZHANG M. Chin. Chem. Lett., 2020, 31(5):1109-1113.
10. [10]
  CHEN C X, ZHAO D, JIANG Y Y, NI P J, ZHANG C H, WANG B, YANG F, LU Y Z, SUN J. Anal. Chem., 2019, 91(23):15017-15024.
11. [11]
  SUN J, ZHAO J H, BAO X F, WANG Q, YANG X R. Anal. Chem., 2018, 90(10):6339-6345.
12. [12]
  MWILU S K, OKELLO V A, OSONGA F J, MILLER S, SADIK O A. Analyst, 2014, 139(21):5472-5481.
13. [13]
  LIN Q S, LI Z H, YUAN Q. Chin. Chem. Lett., 2019, 30(9):1547-1556.
14. [14]
  WANG K, WANG W, ZHANG X Y, JIANG A Q, YANG Y S, ZHU H L. TrAC-Trends Anal. Chem., 2021, 136(2):116189-116218.
15. [15]
  KRIAN S, KHATIK R, SCHIRHAGL S. Anal. Bioanal. Chem., 2019, 411(24):6475-6485.
16. [16]
  LEE M H, KIM J S, SESSLER J L. Chem. Soc. Rev., 2015, 44(13):4185-4191.
17. [17]
  LAD D, BHOSALE S V, JONES L A, BHOSALE S V. ACS Appl. Mater. Interfaces, 2018, 10(15):12189-12216.
18. [18]
  MALASHIKHINA N, GARAI-IBABE G, PAVLOV V. Anal. Chem., 2013, 85(14):6866-6870.
19. [19]
  LIU S, WANG X, PANG S, NA W, YAN X, SU X. Anal. Chim. Acta, 2014, 827:103-110.
20. [20]
  HALAWA M I, GAO W Y, DAQIB M, SHIMELES A K, WU F X, XU G B. Biosens. Bioelectron., 2017, 95:8-14.
21. [21]
  LIU L, JIANG H, WANG X M. Biosens. Bioelectron., 2021, 173:112786-112793.
22. [22]
  HUANG X M, LAN M J, WANG J, GUO L H, LIN Z Y, ZHANG F, ZHANG T, WU C M, QIU B. Anal. Chim. Acta, 2021, 1142:65-72.
23. [23]
  LI J, SI L, BAO J, WANG Z, DAI Z. Anal. Chem., 2017, 89(6):3681-3686.
24. [24]
  XIAO T, SUN J, ZHAO J H, LIU G Y, YANG X R. ACS Appl. Mater. Interfaces, 2018, 10(7):6560-6569.
25. [25]
26. [26]
27. [27]
  KIM T I, KIM H, CHOI Y, KIM Y. Chem. Commun., 2011, 47(35):9825-9827.
28. [28]
  TAN Y, ZHANG L, MAN K H, PELTIER R, CHEN G C, ZHANG H T, ZHOU L Y, WANG F, HO D, YAO S Q, HU Y, SUN H Y. ACS Appl. Mater. Interfaces, 2017, 9(8):6796-6803.
29. [29]
  DONG L, MIAO Q, HAI Z, YUAN Y, LIANG G. Anal. Chem. 2015, 87(13):6475-6478.
30. [30]
  LI Y, SONG H, XUE C, FANG Z, XIONG L, XIE H. Chem. Sci., 2020, 23(11):5889-5894.
31. [31]
  ZHANG P, FU C, ZHANG Q U, LI S, DING C. Anal. Chem., 2019, 91(19):12377-12383.
32. [32]
  ZHAO J H, WANG S, LU S S, SUN J, YANG X R. Nanoscale, 2018, 10(15):7163-7170.
33. [33]
  CHEN Y, LI W Y, WANG Y, YANG X D, CHEN J, JIANG Y N, YU C, LIN Q Y. J. Mater. Chem. C, 2014, 2(20):4080-4085.
34. [34]
  GUO L Y, CHEN D L, YANG M H. Microchim. Acta, 2017, 184(7):2165-2170.
35. [35]
  XIANG M H, LIU J W, LI N, TANG H, LIU R Q, JIANG J H. Nanoscale, 2016, 8(8):4727-4732.
36. [36]
  DENG H H, DENG Q, LI K L, ZHUANG Q Q, ZHUANG Y B, PENG H P, XIA X H, CHEN W. Spectrochim. Acta, Part A, 2020, 229:117875.
37. [37]
  LI Y N, LI Y, WANG X Y, SU X G. New J. Chem., 2014, 38(9):4574-4579.
38. [38]
  YANG Y C, SHIH Y C, HUANG S Y, LU C Y, TSENG W L. Biosens. Bioelectron., 2016, 77:242-248.
39. [39]
  CHEN C X, YUAN Q, NI P J, JIANG Y Y, ZHAO Z L, LU Y Z. Analyst, 2018, 143(16):3821-3828.
40. [40]
  CHEN C X, ZHAO J H, LU Y Z, SUN Y, YANG X R. Anal. Chem., 2018, 90(5):3535-3511.
41. [41]
  GAO M P, WU R Y, MEI Q S, ZHANG C L, LING X, DENG S S, HE H B, ZHANG Y. ACS Sens., 2019, 4(11):2864-2868.
42. [42]
  SUN J, HU T, XU X L, WANG L, YANG X R. Anal. Chem., 2016, 8(38):16846-16850.
43. [43]
  ZHAO D, LI J, PENG C Y, ZHU S Y, SUN J, YANG X R. Anal. Chem., 2019, 91(4):2978-2984.
44. [44]
  PENG C, XING H H, XUE Y, WANG J, LI J, WANG E K. Nanoscale, 2020, 12(3):2022-2027.
45. [45]
  CHEN C X, ZHAO D, WANG B, NI P J, JIANG Y Y, ZHANG C H, YANG F, LU Y Z, SUN J. Anal. Chem., 2020, 92(6):4639-4646.
46. [46]
  LIU G Y, ZHAO J H, YAN M X, ZHU S Y, DOU W C, SUN J, YANG X R. Sci. China Chem., 2020, 63(4):554-560.
47. [47]
  ZHAO J H, WANG S, LU S S, LIU G Y, SUN J, YANG X R. Anal. Chem., 2019, 91(12):7828-7834.
48. [48]
  ZHAO D, CHEN C X, SUN J, YANG X R. Analyst, 2016, 141(11):3280-3288.
49. [49]
  LI G L, FU H L, CHEN X J, GONG P W, CHEN G, XIA L, WANG H, YOU J M, WU Y N. Anal. Chem., 2016, 88(5):2720-2726.
50. [50]
  GUO Z, ZHU X H, WANG S G, LEI C Y, HUANG Y, NIE Z, YAO S Z. Nanoscale, 2018, 10(41):19579-19585.
51. [51]
  HUANGFU X X, SHEN Y, YANG A Z, LIU L X, LUO W, ZHAO W B. Colloids. Surf. B, 2020, 191:110984.
52. [52]
  LIU H, LI M, XIA Y, REN X. ACS Appl. Mater. Interfaces, 2017, 9(1):120-126.
53. [53]
  NI P J, LIU S Y, JIANG Y Y, CHEN C X, WANG B, ZHANG C H, CHEN J B, LU Y Z. ACS Appl. Bio Mater., 2020, 3:6394-6399.
54. [54]
  NI P J, CHEN C X, JIANG Y Y, ZHANG C H, WANG B, LU Y Z, WANG H. Sens. Actuators, B, 2019, 302:127415.
55. [55]
  ZHANG J Y, LU X M, LEI Y, HOU X D, WU P. Nanoscale, 2017, 9(4):15606-15611.
56. [56]
  CHEN C X, ZHANG G L, NI P J, JIANG Y Y, LU Y Z, LU Z L. Microchim. Acta, 2019, 186(6):348.
57. [57]
  NI P J, CHEN C X, JIANG Y Y, ZHANG C H, WANG B, CAO B, LI C, LU Y Z. Sens. Actuators, B, 2019, 301:127080.
58. [58]
  ZHU R F, HUANG W Y, MA X F, ZHANG Y H, YUE C C, FANG W H, HU Y, WANG J, DANG J Q, ZHAO H, LI Z X. Anal. Chim. Acta, 2019, 1089:131-143.
59. [59]
60. [60]
  NA W D, LI N, SU X G. Sens. Actuators, B, 2018, 274:172-179.
61. [61]
  LIANG M Y, ZHAO B, XIONG Y, CHEN W X, HUO J Z, ZHANG F, WANG L, LI Y. Dalton Trans., 2019, 48(43):16199-16210.
62. [62]
  QU F L, PEI H M, KONG R M, ZHU S Y, XIA L. Talanta, 2017, 165:136-142.
63. [63]
  LIU J J, TANG D S, CHEN Z T, YAN X M, ZHONG Z, KANG L T, YAO J N. Biosens. Bioelectron., 2017, 94:271-277.
64. [64]
  HAN X, MENG Z, LI X L, QU F L, KONG R M. Talanta, 2020, 212:120768-120775.
65. [65]
  ZHANG Q F, ZHANG C Y, SHAHZAD S A, YU C. Talanta, 2016, 158:342-350.
66. [66]
  LIU S G, HAN L, LI N, XIAO N, JU J Y, LI N B, LUO H Q. J. Mater. Chem. B, 2018, 6(18):2843-2850.
67. [67]
  YANG Q, LI C Y, LI J H, ARABI M, WANG X Y, PENG H L, XIONG H, CHOO J, CHEN L X. J. Mater. Chem. C, 2020, 8(16):5554-5561.
68. [68]
  NI P J, XIE J F, CHEN C X, JIANG Y Y, LU Y Z, HU X. Microchim. Acta, 2019, 186:202-208.
69. [69]
  SHI F P, LI J, SUN J J, HUANG H, SU X G, WANG Z H. Talanta, 2020, 207:120341.
1. [1]
  Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
2. [2]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
3. [3]
  Xiaofei NIU , Ke WANG , Fengyan SONG , Shuyan YU . Self-assembly of [Pd₆(L)₄]⁸⁺-type macrocyclic complexes for fluorescent sensing of HSO₃^-. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1233-1242. doi: 10.11862/CJIC.20240057
4. [4]
  Lei ZHANG , Cheng HE , Yang JIAO . An azo-based fluorescent probe for the detection of hypoxic tumor cells. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1162-1172. doi: 10.11862/CJIC.20250081
5. [5]
  Rui TIAN , Duo LI , Yuan REN , Jiamin CHAI , Xuehua SUN , Haoyu LI , Yuecheng ZHANG . Dual-ligand-modified copper nanoclusters: Synthesis and application in ornidazole detection. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1245-1255. doi: 10.11862/CJIC.20240389
6. [6]
  Xuehua SUN , Min MA , Jianting LIU , Rui TIAN , Hongmei CHAI , Huali CUI , Loujun GAO . Pr/N co-doped biomass carbon dots with enhanced fluorescence for efficient detection of 2,4-dinitrophenylhydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 561-573. doi: 10.11862/CJIC.20240294
7. [7]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
8. [8]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
9. [9]
  Yingpeng ZHANG , Xingxing LI , Yunshang YANG , Zhidong TENG . A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1301-1308. doi: 10.11862/CJIC.20250064
10. [10]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
11. [11]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
12. [12]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
13. [13]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
14. [14]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
15. [15]
  Zhonghua Xi , Xuanfeng Kong , Jinyue Yang , Bin Liu , Tingyu Zhu , Hui Zhang , Wenwei Zhang . Construction of Public Teaching Instrument Platform and Exploration of Opening Mechanism. University Chemistry, 2024, 39(7): 200-206. doi: 10.12461/PKU.DXHX202405123
16. [16]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
17. [17]
  Baohua LÜ , Yuzhen LI . Anisotropic photoresponse of two-dimensional layered α-In₂Se₃(2H) ferroelectric materials. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1911-1918. doi: 10.11862/CJIC.20240105
18. [18]
  Xinzhe HUANG , Lihui XU , Yue YANG , Liming WANG , Zhangyong LIU , Zhongjian WANG . Preparation and visible light responsive photocatalytic properties of BiSbO₄/BiOBr. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 284-292. doi: 10.11862/CJIC.20240212
19. [19]
  Yan ZHAO , Xiaokang JIANG , Zhonghui LI , Jiaxu WANG , Hengwei ZHOU , Hai GUO . Preparation and fluorescence properties of Eu³⁺-doped CaLaGaO₄ red-emitting phosphors. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1861-1868. doi: 10.11862/CJIC.20240242
20. [20]
  Huan LI , Shengyan WANG , Long Zhang , Yue CAO , Xiaohan YANG , Ziliang WANG , Wenjuan ZHU , Wenlei ZHU , Yang ZHOU . Growth mechanisms and application potentials of magic-size clusters of groups Ⅱ-Ⅵ semiconductors. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1425-1441. doi: 10.11862/CJIC.20240088

Get Citation

PDF

XML

Metrics

PDF Downloads(32)
Abstract views(1156)
HTML views(235)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142