Citation: GUO Jia, WEI Li-Ping, CHEN Zhi-Ying, HE Cheng-Yan. Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

1.
Department of Respiratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
Department of Clinical Laboratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China

Corresponding author: CHEN Zhi-Ying, HE Cheng-Yan,
Received Date: 26 June 2021
Revised Date: 5 August 2021

Fund Project: Supported by the Jilin Province Science and Technology Department, China (Nos.20190201215JC, 20200404193YY).

The abnormal expression of protein acyltransferase that is responsible for protein palmitoylation in renal clear cell carcinoma suggests that there may be abnormal changes of S-palmitoylation in renal clear cell carcinoma, which is closely related to metabolic disorder or activation of carcinogenic signaling pathway. In the present study, the optimized acyl-biotinyl exchange (ABE) method combined with proteomic techniques was used to analyze S-palmitoylated proteins in clear cell renal cell carcinoma. A total of 92 palmitoylated proteins were identified with high reliability, and the S-palmitoylation of adipose differentiation related protein (ADRP) was verified by Western blotting. This study provides an improved ABE method suitable for analyzing tissue samples. The identification of palmitoylated proteins in clear cell renal cell carcinoma can provide primary data for the molecular targets of early diagnosis, treatment and prognosis of clear cell renal carcinoma, which are of great significance for further study of the pathogenesis of clear cell renal carcinoma.
- S-palmitoylation,
- Clear cell renal cell carcinoma,
- Proteome,
- Acyl-biotin exchange,
- Adipose differentiation related proteins
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沈阳化工大学材料科学与工程学院沈阳 110142