Citation: LU Wen-Bo, BAO Can-Can, WEI Ming, NIU Qiang-Qiang, BAI Li-Wei, CAO Xiao-Wei, JIA Jian-Feng. An Electrochemical Immunosensor Based on Gold Nanoparticles Decorated Cobalt Boride Nanosheet Arrays for Detection of Human Chorionic Gonadotropin[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2075-2085. doi: 10.19756/j.issn.0253-3820.210543 shu

An Electrochemical Immunosensor Based on Gold Nanoparticles Decorated Cobalt Boride Nanosheet Arrays for Detection of Human Chorionic Gonadotropin

1.
Key Laboratory of Magnetic Molecules and Magnetic Information, Materials Ministry of Education, School of Chemistry and Material Science, Shanxi Normal University, Taiyuan 030035, China;
2.
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225009, China

Corresponding author: LU Wen-Bo, luwb@sxnu.edu.cn
Received Date: 3 June 2021
Revised Date: 30 July 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21705103), the Applied Basic Research Project of Shanxi Province, China (No.201801D221392), the Scientific and Technological Innovation Projects in Shanxi Universities (No.2019L0460) and the 1331 Engineering of Shanxi Province, China.

Human chorionic gonadotropin (hCG) is a marker of early pregnancy and diseases such as ovarian cancer. Therefore, the rapid, accurate and real-time determination of hCG is of great significance. In this work, an electrochemical immunosensor for detection of hCG based on three-dimensional Au nanoparticles anchored on cobalt boride (AuNPs/CoB) nanosheet array electrode was developed. AuNPs/CoB nanosheet arrays were synthesized by an electrodeposition method, which was first explored for immunoassay of hCG. AuNPs/CoB nanosheeet arrays could enhance the performance of electrocatalytic reduction of thionine to amplify signal. Thionine (Thi) was electro-polymerized on the AuNPs/CoB nanosheet arrays, thus forming Thi/AuNPs/CoB nanosheet array-electrode. HCG antibody (anti-hCG) could be successfully attached to the Thi/AuNPs/Co-B nanosheet array-electrode by Au-N bond of AuNPs or the amino group of thionine, which caused an obvious decrease in the catalytic current due to the insulated anti-hCG. The current signal decreased linearly with the increase of hCG concentration due to the formation of antibody-antigen immunocomplex. The immunosensor possessed a good linearity with logarithm of hCG concentration in the range of 10-5.0×10⁴ pg/mL, and the detection limit was 2.80 pg/mL (S/N=3). The immunosensor based on Thi/AuNPs/Co-B nanosheet arrays could exactly detect hCG in human serum samples, providing a simple and reliable evaluation method for the clinical diagnosis of early pregnancy and gynecological diseases.
- Cobalt boride nanosheet,
- Array,
- Gold nanoparticles,
- Human chorionic gonadotropin,
- Electrochemical immunosensor
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