Citation: MA Yi, QI Xie-Min, WU Hai-Ping, MA Xue-Ping, CHU Ya-Nan, WANG Xue-Mei, ZHOU Guo-Hua. Research Progress in Paper-based Biosensors for Pathogen Nucleic Acids[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 1945-1954. doi: 10.19756/j.issn.0253-3820.210465 shu

Research Progress in Paper-based Biosensors for Pathogen Nucleic Acids

MA Yi ¹ ,
QI Xie-Min ¹ ,
WU Hai-Ping ^1,2 ,
MA Xue-Ping ¹ ,
CHU Ya-Nan ¹ ,
WANG Xue-Mei ² ,
ZHOU Guo-Hua ^1,2,,

1.
Department of Clinical Pharmacy, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China;
2.
School of Pharmacy, Southern Medical University, Nanjing 210002, China

Corresponding author: ZHOU Guo-Hua, ghzhou@nju.edu.cn
Received Date: 25 April 2021
Revised Date: 20 June 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.61871403), the Jiangsu Provincial Science Fund for Distinguished Young Scholars (No.BK20180005) and the Jiangsu Provincial Medical Youth Talent Program (Nos.QNRC2016889, QNRC2016845).

Infectious diseases caused by pathogens seriously threaten human life and health. Rapid and accurate detection of pathogens is an important prerequisite for effective prevention and control of infectious diseases. However, traditional methods for detection of pathogen nucleic acid often require professional personnel and specialized instruments and equipments, which challenges the realization of field detection of pathogen. Using paper as matrix to construct biosensor has the advantages of light and portable, low cost and intuitive results, and is suitable for rapid detection of pathogens in the field by combining with nucleic acid amplification method. Currently, the paper-based pathogen nucleic acid detection technique mainly includes two types:lateral flow strips and paper-based microfluidic devices. Here, antibody-dependent and antibody-independent lateral flow strips, two- and three-dimensional paper-based microfluidic devices and fabrication techniques are reviewed, and the strategies of developing paper-based integrated devices for nucleic acid extraction, amplification and detection of pathogens are introduced, which can provide a reference for the development of new techniques for the field detection of pathogens.
- Lateral flow strip,
- Microfluidic paper-based analytical device,
- Paper-based integrated device,
- Pathogen,
- Nucleic acid detection,
- Review
1. [1]
  CHOI Y J, HWANG J H, LEE S Y. Small Methods, 2018, 2(4):1700351.
2. [2]
  ZHOU P, YANG X L, WANG X G, HU B, ZHANG L, ZHANG W, SI H R, ZHU Y, LI B, HUANG C L, CHEN H D, CHEN J, LUO Y, GUO H, JIANG R D, LIU M Q, CHEN Y, SHEN X R, WANG X, ZHENG X S, ZHAO K, CHEN Q J, DENG F, LIU L L, YAN B, ZHAN F X, WANG Y Y, XIAO G F, SHI Z L. Nature, 2020, 579(7798):270-273.
3. [3]
  CHAN J F, YUAN S, KOK K H, TO K K, CHU H, YANG J, XING F, LIU J, YIP C C, POON R W, TSOI H W, LO S K, CHAN K H, POON V K, CHAN W M, IP J D, CAI J P, CHENG V C, CHEN H, HUI C K, YUEN K Y. Lancet, 2020, 395(10223):514-523.
4. [4]
  ZHU N, ZHANG D, WANG W, LI X, YANG B, SONG J, ZHAO X, HUANG B, SHI W, LU R, NIU P, ZHAN F, MA X, WANG D, XU W, WU G, GAO G F, TAN W. N. Engl. J. Med., 2020, 382(8):727-733.
5. [5]
  WANG C, HORBY P W, HAYDEN F G, GAO G F. Lancet, 2020, 395(10223):470-473.
6. [6]
  JIANG S, SHI Z, SHU Y, SONG J, GAO G F, TAN W, GUO D. Lancet, 2020, 395(10228):949.
7. [7]
  ZOUMPOURLIS V, GOULIEMAKI M, RIZOS E, BALIOU S, SPANDIDOS D A. Mol. Med. Rep., 2020,22(4):3035-3048.
8. [8]
  DAWOOD F S, JAIN S, FINELLI L, SHAW M W, LINDSTROM S, GARTEN R J, GUBAREVA L V, XU X, BRIDGES C B, UYEKI T M. N. Engl. J. Med., 2009, 360(25):2605-2615.
9. [9]
  ZHONG N S, ZHENG B J, LI Y M, POON L L M, XIE Z H, CHAN K H, LI P H, TAN S Y, CHANG Q,XIE J P, LIU X Q, XU J, LI D X,YUEN K Y, PEIRIS J S M, GUAN Y. Lancet, 2003, 362(9393):1353-1358.
10. [10]
  DWIVEDI H P, JAYKUS L A. Crit. Rev. Microbiol., 2011, 37(1):40-63.
11. [11]
  STEPHANIE D G, KRUTI R P. J. Invest. Dermatol., 2013, 133(e12):1-3.
12. [12]
  SALLY A B, MICHAEL C, MICHA N. Transfus. Med. Hemother., 2015, 42(4):211-218.
13. [13]
  KLING C E, PERKINS J D, LANDIS C S, LIMAYE A P, SIBULESKY L. Am. J. Transplant., 2017, 17(11):2863-2868.
14. [14]
  CHEN H, LIU K K, LI Z, WANG P. Clin. Chim. Acta, 2019, 493:138-147.
15. [15]
  PEAPER D R, DURANT T, CAMPBELL S. Clin. Lab Med., 2019, 39(3):419-431.
16. [16]
  POSCHENRIEDER A, THALER M, JUNKER R, LUPPA P B. Anal. Bioanal. Chem., 2019, 411(29):7607-7621.
17. [17]
  MARTINEZ A W, PHILLIPS S T, BUTTE M J, WHITESIDES G M. Angew. Chem., Int. Ed., 2007, 46(8):1318-1320.
18. [18]
  CATE D M, ADKINS J A, METTAKOONPITAK J, HENRY C S. Anal. Chem., 2015, 87(1):19-41.
19. [19]
  YETISEN A K, AKRAM M S, LOWE C R. Lab Chip, 2013, 13:2210-2251.
20. [20]
  BAHADIR E B, SEZGINTURK M K. TrAC-Trends Anal. Chem., 2016, 82:286-306.
21. [21]
  BATULE B S, SEOK Y, KIM M G. Biosens. Bioelectron., 2020, 151:111998.
22. [22]
  JIANG X, LOEB J C, PAN M, FAN Z H. Anal. Chim. Acta, 2021, 1165:338542.
23. [23]
  LEUVEING J H, THAL P J, VAN DER WAART M, SCHUURS A H. J. Immunol. Methods, 1981, 45:183-194.
24. [24]
  KAINZ D M, FRVH S M, HUTZENLAUB T, ZENGERLE R, PAUST N. Lab Chip, 2019, 19(16):2718-2727.
25. [25]
  KIM S H, LEE J, LEE B H, SONG C S, GU M B. Biosens. Bioelectron., 2019, 134:123-129.
26. [26]
  WANG X K, WANG X D, SHI C, MA C P, CHEN L X. Talanta, 2020, 216:120978.
27. [27]
28. [28]
  LUO K, KIM H Y, OH M H, KIM Y R. Crit. Rev. Food Sci. Nutr., 2020, 60(1):157-170.
29. [29]
  TAKABATAKE R, KAGIYA Y, MINEGISHI Y, FUTO S, SOGA K, NAKAMURA K, KONDO K, MANO J, KITTA K. J. Agric. Food Chem., 2018, 66(29):7839-7845.
30. [30]
  YANG M, MUDABUKA B, QUIZON K, NFON C. Transbound. Emerg. Dis., 2019, 66(3):1158-1166.
31. [31]
  JUNG J H, OH S J, KIM Y T, KIM S Y, KIM W J, JUNG J, SEO T S. Anal. Chim. Acta, 2015, 853:541-547.
32. [32]
  HUANG P, WANG H, CAO Z, JIN H, CHI H, ZHAO J, YU B, YAN F, HU X, WU F, JIAO C, HOU P, XU S, ZHAO Y, FENG N, WANG J, SUN W, WANG T, GAO Y, YANG S, XIA X. Front. Microbiol., 2018, 9:1101-1110.
33. [33]
  GOOTENBERG J S, ABUDAYYEH O O, KELLNER M J, JOUNG J, COLLINS J J, ZHANG F. Science, 2018, 360(6387):439-444.
34. [34]
  MYHRVOLD C, FREIJE C A, GOOTENBERG J S, ABUDAYYEH O O, METSKY H C, DURBIN A F, KELLNER M J, TAN A L, PAUL L M, PARHAM L A, GARCIA K F, BARNES K G, CHAK B, MONDINI A, NOGUEIRA M L, ISERN S, MICHAEL S F, LORENZANA I, YOZWIAK N L, MACINNIS B L, BOSCH I, GEHRKE L, ZHANG F, SABETI P C. Science, 2018, 360(6387):444-448.
35. [35]
  ABUDAYYEH O O, GOOTENBERG J S, KONERMANN S, JOUNG J, SLAYMAKER I M, COX D B T, SHMAKOV S, MAKAROVA K S, SEMENOVA E, MINAKHIN L, SEVERINOV K, REGEV A, LANDER E S, KOONIN E V, ZHANG F. Science, 2016, 353(6299):aaf5573.
36. [36]
  SMARGON A A, COX D B T, PYZOCHA N K, ZHENG K, SLAYMAKER I M, GOOTENBERG J S, ABUDAYYEH O A, ESSLETZBICHLER P, SHMAKOV S, MAKAROVA K S, KOONIN E V, ZHANG F. Mol. Cell, 2017, 65(4):618-630.
37. [37]
  CHEN J S, MA E, HARRINGTON L B, COSTA M D, TIAN X R, PALEFSKY J M, DOUDNA J A. Science, 2018, 360(6387):436-439.
38. [38]
  BROUGHTON J P, DENG X, YU G, FASCHING C L, SERVELLITA V, SINGH J, MIAO X, STREITHORST J A, GRANADOS A, SOTOMAYOR GONZALEZ A, ZOM K, GOPEZ A, HSU E, GU W, MILLER S, PAN C Y, GUEVARA H, WADFORD D A, CHEN J S, CHIU C Y. Nat. Biotechnol., 2020, 38(7):870-874.
39. [39]
  ZHANG F, ABUDAYYEH O O, GOOTENBERG J S. (2020-03-21)[2021-04-24], https://broad.io/sherlockprotocol.
40. [40]
  PATCHSUNG M, JANTARUG K, PATTAMA A, APHICHO K, SURARITDECHACHAI S, MEESAWAT P, SAPPAKHAW K, LEELAHAKORN N, RUENKAM T, WONGSATIT T, ATHIPANYASILP N, EIAMTHONG B, LAKKANASIRORAT B, PHOODOKMAI T, NILJIANSKUL N, PAKOTIPRAPHA D, CHANARAT S, HOMCHAN A, TINIKUL R, KAMUTIRA P, PHIWKAOW K, SOITHONGCHAROEN S, KANTIWIRIYAWANITCH C, PONGSUPASA V, TRISRIVIRAT D, JAROENSUK J, WONGNATE T, MAENPUEN S, CHAIYEN P, KAMNERDNAKTA S, SWANGSRI J, CHUTHAPISITH S, SIRIVATANAUKSORN Y, CHAIMAYO C, SUTTHENT R, KANTAKAMALAKUL W, JOUNG J, LADHA A, JIN X, GOOTENBERG J S, ABUDAYYEH O O, ZHANG F, HORTHONGKHAM N, UTTAMAPINANT C. Nat. Biomed. Eng., 2020, 4(12):1140-1149.
41. [41]
  AVEYARD J, MEHRABI M, COSSINS A, BRAVEN H, WILSON R. Chem. Commun., 2007, (41):4251-4253.
42. [42]
  MONDEN Y, TAKASAKI K, FUTO S, NIWA K, KAWASE M, AKITAKE H, TAHARA M. J. Biotechnol., 2014, 185:57-62.
43. [43]
  TIAN L Y, SATO T, NIWA K, KAWASE M, TANNER A C R, NOBUHIRO T. BioMed. Res. Int., 2014, 2014:180323.
44. [44]
  TAKAEADA Y, KODERA T, KOBAYASHI K, NAKAJIMA C, KAWASE M, SUZUKI Y. J. Microbiol. Methods, 2020, 177:106062.
45. [45]
  YANG Y, NOVIANA E, NGUYEN M P, GEISS B J, DANDY D S, HENRY C S. Anal. Chem., 2017, 89(1):71-91.
46. [46]
  CATE D M, ADKINS J A, METTAKOONPITAK J, HENRY C S. Anal. Chem., 2015, 87(1):19-41.
47. [47]
  CHINNADAYYALA S R, PARK J, LE H T N, SANTHOSH M, KADAM A N, CHO S. Biosens. Bioelectron., 2019, 126:68-81.
48. [48]
  BEDIN F, BOULET L, VOILIN E, THEILLET G, RUBENS A, ROZAND C. J. Med. Virol., 2017, 89(9):1520-1527.
49. [49]
  HU J, WANG S Q, WANG L, LI F, PINGGUAN-MURPHY B, LU T J, XU F. Biosens. Bioelectron., 2014, 54:585-597.
50. [50]
  WHITESIDES G M. Nature, 2006, 442(7101):368-373.
51. [51]
  REN K, ZHOU J, WU H. Acc. Chem. Res., 2013, 46(11):2396-406.
52. [52]
  NOVIANA E, MCCORD C P, CLARK K M, JANG I, HENRY C S. Lab Chip, 2020, 20(1):9-34.
53. [53]
  HORST A L, ROSENBOHM J M, KOLLURI N, HARDICK J, GAYDOS C A, CABODI M, KLAPPERICH C M, LINNES J C. Biomed. Microdevices, 2018, 20(2):35-49.
54. [54]
  LI X, BALLERINI D R, SHEN W. Biomicrofluidics, 2012, 6(1):011301.
55. [55]
  MORBIOLI G G, MAZZU-NASCIMENTO T, STOCKTON A M, CARRILHO E. Anal. Chim. Acta, 2017, 970:1-22.
56. [56]
  MORA M F, GARCIA C D, SCHAUMBURG F, KLER P A, BERLI C L A, HASHIMOTO M, CARRILHO E. Anal. Chem., 2019, 91(13):8298-8303.
57. [57]
  ASANO H, SHIRAISHI Y. Anal. Chim. Acta, 2015, 883:55-60.
58. [58]
  RAJ N, BREEDVELD V, HESS D W. Lab Chip, 2019, 19(19):3337-3343.
59. [59]
  DE OLIVEIRA T R, FONSECA W T, DE OLIVEIRA SETTI G, FARIA R C. Talanta, 2019, 195:480-489.
60. [60]
  GHOSH R, GOPALAKRISHNAN S, SAVITHA R, RENGANATHAN T, PUSHPAVANAM S. Sci. Rep., 2019, 9:7896.
61. [61]
  CHIANG C K, KURNIAWAN A, KAO C Y, WANG M J. Talanta, 2019, 194:837-845.
62. [62]
  DUNGCHAI W, CHAILAPAKUL O, HENRY C S. Analyst, 2011, 136(1):77-82.
63. [63]
  SONGOK J, TOIVAKKA M. ACS Appl. Mater. Interfaces, 2016, 8(44):30523-30530.
64. [64]
  ROBERT B C, MICHAEL P N, ALEXIS G S, ELIJAH M H, JOHN V, DAVID S D, CHARLES S H. Lab Chip, 2018, 18:793-802.
65. [65]
  SOUM V, PARK S, BRILIAN A I, CHOI J Y, LEE Y W, KIM W J, KWONA O S, SHIN K. Lab Chip, 2020, 20:1601-1611.
66. [66]
  KAARJ K, AKARAPIPAD P, YOON J Y. Sci. Rep., 2018, 8:21438.
67. [67]
  KAUSHIK A, TIWARI S, DEV JAYANT R, NAIR M. Biosens. Bioelectron., 2016, 75:254-272.
68. [68]
  XIA Y, SI J, LI Z. Biosens. Bioelectron., 2016, 77:774-789.
69. [69]
  TEENGAM P, SIANGPROH W, TUANTRANONT A, VILAIVAN T, CHAILAPAKUL O, HENRY C S. Anal. Chem., 2017, 89(10):5428-5435.
70. [70]
  CHOI J R, HU J, TANG R H, GONG Y, FENG S S, REN H, WEN T, LI X, ABAS W A B W, MURPHY B P, XU F. Lab Chip, 2016, 16:611-621.
71. [71]
  RODRIGUEZ N M, WONG W S, LIU L, DEWAR R, KLAPPERICH C M. Lab Chip, 2016, 16:753-763.
72. [72]
  XU G L, NOLDER D, REBOUD J, OGUIKE M C, SCHALKWYK D A, SUTHERLAND C J, COOPER J M. Angew. Chem., Int. Ed., 2016, 55(49):15250-15253.
73. [73]
  REBOUD J, XU G L, GARRETT A, ADRIKO M, YANG Z G, TUKAHEBWA E M, ROWELL C, COOPER J M. Proc. Natl. Acad. Sci. U. S. A., 2019, 116(11):4834-4842.
74. [74]
  MAGRO L, JACQUELIN B, ESCADAFAL C, GARNERET P, KWASIBORSKI A, MANUGUERRA J C, MONTI F, SAKUNTABHAI A, VANHOMWEGEN J, LAFAYE P. Sci. Rep., 2017, 7:1347.
75. [75]
  AHN H, BATULE B S, SEOK Y, KIM M G. Anal. Chem., 2018, 90(17):10211-10216.
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