Citation: ZHANG Rui-Xue, CHEN Wen-Jing, JIANG Wei, XU Xiao-Wen. Construction of Telomerase Activity Test Strip and Visual Determination of Cancerous Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(5): 702-709. doi: 10.19756/j.issn.0253-3820.201765 shu

Construction of Telomerase Activity Test Strip and Visual Determination of Cancerous Cells

School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China

Corresponding author: XU Xiao-Wen, xuxw@sdu.edu.cn
Received Date: 17 December 2020
Revised Date: 17 January 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21705094), the Open Funds of the State Key Laboratory of Electroanalytical Chemistry (No.SKLEAC202004), and the Young Scholars Program of Shandong University (No.2018WLJH50).

Telomerase is a ribonucleoprotein that can catalyze the addition of telomerase repeats onto the end of telomere, compensating for the telomere shortening during cell division and maintaining the continuous cell proliferation. Telomerase activity is overexpressed in cancerous cells but inhibited in normal somatic cells, and thus it is regarded as an important biomarker for cancer diagnosis and prognosis evaluation. In this work, a test strip is constructed for visual detection of telomerase activity and determination of cancerous cells. The test strip is constituted of sample pad, conjugate pad that is sprayed with DNA-functionalized gold nanoparticles, nitrocellulose membrane that contains test zone and control zone, and absorbent pad, which are all assembled onto the backing plate. Under the action of telomerase, the substrate sequence gets extended, which are then dropped onto the sample pad. The solution migrates by capillary action and rehydrates the DNA-functionalized AuNPs on the conjugate pad. Subsequently, the telomeric products hybridize with the capture DNA in the test zone and the modified DNA on AuNPs in a sandwich manner, causing the accumulation of AuNPs on the test zone and the color development of T-line. Meanwhile, the DNA-functionalized AuNPs continue flowing and then hybridize with the capture DNA on the control zone, causing the color development of C-line and confirming the validity of the test strip. Through responding to the telomerase from cell extracts, the method can achieve the visual differentiation of cancerous cells from normal cells in a convenient and rapid way by observation of color development of T-line.
- Telomerase,
- Test strip,
- Cancerous cells,
- Visual detection
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