Citation: WANG Dan, QIU Wan-Ya, YU Shi-Yu, LIU Zhi-Min, ZHANG Xue-Hua, XU Zhi-Gang, ZHU Rui-Zhi, ZHANG Feng-Mei, SI Xiao-Xi, LIU Zhi-Hua. Simultaneous Analysis of Ascorbic Acid, Uric Acid and Xanthine in Human Urine by Column Tandem Technique[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1919-1925. doi: 10.19756/j.issn.0253-3820.201754 shu

Simultaneous Analysis of Ascorbic Acid, Uric Acid and Xanthine in Human Urine by Column Tandem Technique

1.
Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China;
2.
R&D Center of China Tobacco Yunnan Industrial Co. Ltd., Kunming 650231, China

Corresponding author: XU Zhi-Gang, SI Xiao-Xi,
Received Date: 12 December 2020
Revised Date: 24 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21565018), the China National Tobacco Corporation Science and Technology Major Special Project (No.110201901002(XX-02)) and the Yunnan China Tobacco Industry Co., Ltd. Key Project (No.2018JC05).

Ascorbic acid, uric acid and xanthine in human urine are important markers of many diseases. A method for simultaneous detection of ascorbic acid, uric acid and xanthine in human urine by high performance liquid chromatography based on column tandem technique was established. The separation of these three compounds was the key to simultaneously analyze the three compounds to a certain extent because the conventional liquid chromatography could not effectively separate these highly polar substances simultaneously. The Innoval ASB C₁₈ was coupled with the hydrophilic interaction chromatography (HILIC) column to trigger off a dual-column series separation mode. The separation performance of the two columns was complementary to achieve baseline separation of the three target analytes. The results showed that a single HILIC column could not achieve the baseline separation of the three analytes. The C₁₈ column also showed very weak retention to the three analytes. The retention time was so early that the target analyte peaks might be interfered by the solvent peaks. Only the tandem of these two columns could achieve the baseline separation for the three analytes. Ultimately, an analytical method for simultaneous analysis of ascorbic acid, uric acid and xanthine in human urine with the C₁₈ column tandem HILIC column was established. The correlation coefficient was good in the linear range of 0.005-20 mg/L, and limits of detection (LODs) of ascorbic acid, uric acid, and xanthine were 1.06 μg/L, 0.94 μg/L and 0.46 μg/L, respectively. The three target analytes could be found in actual human urine by the proposed method. The contents of ascorbic acid, uric acid and xanthine were 0.47-4.70, 6.91-8.01 and 0.10-0.19 mg/L, respectively. The recoveries of the spiked urine samples were 86.2%-117.9%, and the relative standard deviation (RSD) was less than 9.3%. The established high performance liquid chromatography based on column tandem analytical method was simple, fast and efficient, and could be used for separation and simultaneous determination of ascorbic acid, uric acid and xanthine in human urine.
- Column tandem technique,
- Hydrophilic interaction chromatography column,
- C₁₈ column,
- Ascorbic acid,
- Uric acid,
- Xanthine
1. [1]
  HUSSAIN M M, ASIRI A M, RAHMAN M M. Microchem. J., 2020, 159:105534.
2. [2]
  ABELLAN-LLOBREGAT A, VIDAL L, RODRÍGUEZ-AMARO R, BERENGUER-MURCIA A, CANALS A, MORALLÓN E. Electrochim. Acta, 2017, 227:275-284.
3. [3]
  SEN S, SARKAR P. Anal. Chim. Acta, 2020, 1114:15-28.
4. [4]
  JINDAL K, TOMAR M, GUPTA V. Biosens. Bioelectron., 2014, 55(9):57-65.
5. [5]
  FEIG D I, KANG D H, JOHNSON R J. N. Engl. J. Med., 2008, 359(17):1811-1821.
6. [6]
  IBRAHIM H, TEMERK Y. J. Electroanal. Chem., 2016, 780:176-186.
7. [7]
  WANG X X, WU Q, SHAN Z, HUANG Q M. Biosens. Bioelectron., 2011, 26(8):3614-3619.
8. [8]
  WESTLEY C, XU Y, THILAGANATHAN B, CARNELL A J, TURNER N J, GOODACRE R. Anal. Chem., 2017, 89(4):2472-2477.
9. [9]
  FOWLER A A, SYED A A, KNOWLSON S, SCULTHORPE R, FARTHING D, DEWILDE C, FARTHING C A, LARUS T L, MARTIN E, BROPHY D F, GUPTA S, RESPIRATORY M, NURSING I C U, FISHER B J, NATARAJAN R. J. Transl. Med., 2014, 12(1):32.
10. [10]
11. [11]
  ZHANG F Y, WANG Z H, ZHANG Y Z, ZHENG Z X, WANG C M, DU Y L, YE W H. Talanta, 2012, 93:320-325.
12. [12]
  HASOÑ S, OSTATNÁ V, FOJTA M. Electrochim. Acta, 2020, 329:135132.
13. [13]
14. [14]
  BADOEI-DALFARD A, SOHRABI N, KARAMI Z, SARGAZI G. Biosens. Bioelectron., 2019, 141:111420.
15. [15]
  COOPER N, KHOSRAVAN R, ERDMANN C, FIENE J, LEE J W. J. Chromatogr. B, 2006, 837(1-2):1-10.
16. [16]
17. [17]
18. [18]
  SUN D, ZHANG Y, WANG F R, WU K B, CHEN J W, ZHOU Y K. Sens. Actuators, B, 2009, 141(2):641-645.
19. [19]
  ABELLÁN-LLOBREGAT A, VIDAL L, RODRÍGUEZ-AMARO R, BERENGUER-MURCIA Á, CANALS A, MORALLÓN E. Electrochim. Acta, 2017, 227:275-284.
20. [20]
  PLESKACOVA A, BREICHA S, PACAL L, KANKOVA K, TOMANDL J.Chromatographia, 2017, 80:529-536.
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
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