Citation: ZHANG Tong, TAO Qing, BIAN Xiao-Jun, CHEN Qian, YAN Juan. Rapid Visualized Detection of Escherichia Coli O157: H7 by DNA Hydrogel Based on Rolling Circle Amplification[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(3): 377-386. doi: 10.19756/j.issn.0253-3820.201744 shu

Rapid Visualized Detection of Escherichia Coli O157: H7 by DNA Hydrogel Based on Rolling Circle Amplification

ZHANG Tong ¹ ,
TAO Qing ¹ ,
BIAN Xiao-Jun ^1,2,3 ,
CHEN Qian ¹ ,
YAN Juan ^1,2,3,,

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

Corresponding author: YAN Juan, j-yan@shou.edu.cn
Received Date: 8 December 2020
Revised Date: 13 January 2021

Fund Project: National Natural Science Foundation of China (No. 21775102) and the Natural Science Foundation of Shanghai Municipal, China (No. 20ZR1424100)

Foodborne illnesses caused by pathogenic bacteria are prominent issues in food safety. Fast and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptasensor based on aldehyde magnetic beads, rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia Coli O157:H7 (E.coli O157:H7). The Mbs@dsDNA complexes were first prepared. In the presence of target, the aptamer combined with E.coli O157:H7, which released E.coliaptamer-initiator. The supernatant collected by magnetic separation and the released primers were hybridized with circular sequence to form padlock probe. Then, the RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase and dNTPs, while the RCA could not be triggered in the absence of target. Those two circular sequences were designed to contain partially complementary bases. Thus, when those two circular sequences generated two long single-stranded DNA (ssDNA) products respectively after RCA reaction, two ssDNA products hybridized with each other and formed naked-eye visible DNA hydrogel. The aptasensor was successfully used for visualized detection of E.coli O157:H7 with a detection limit of 4×10³ CFU/mL, and the detection could be finished within 30 min. The aptasensor had many advantages such as high specificity and specificity, easy operation, efficient amplification, and rapid and visualized readout, showing potential in applications of food safety detection.
- Rolling circle amplification,
- DNA hydrogel,
- Aptamer,
- Food-borne illness,
- Food safety
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
1. [1]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
2. [2]
  Wei Li , Jinfan Xu , Yongjun Zhang , Ying Guan . 共价有机框架整体材料的制备及食品安全非靶向筛查应用——推荐一个仪器分析综合化学实验. University Chemistry, 2025, 40(6): 276-285. doi: 10.12461/PKU.DXHX202406013
3. [3]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
4. [4]
  Yuena Yang , Xufang Hu , Yushan Liu , Yaya Kuang , Jian Ling , Qiue Cao , Chuanhua Zhou . The Realm of Smart Hydrogels. University Chemistry, 2024, 39(5): 172-183. doi: 10.3866/PKU.DXHX202310125
5. [5]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
6. [6]
  Qiang Zhou , Pingping Zhu , Wei Shao , Wanqun Hu , Xuan Lei , Haiyang Yang . Innovative Experimental Teaching Design for 3D Printing High-Strength Hydrogel Experiments. University Chemistry, 2024, 39(6): 264-270. doi: 10.3866/PKU.DXHX202310064
7. [7]
  Qingyang Cui , Feng Yu , Zirun Wang , Bangkun Jin , Wanqun Hu , Wan Li . From Jelly to Soft Matter: Preparation and Properties-Exploring of Different Kinds of Hydrogels. University Chemistry, 2024, 39(9): 338-348. doi: 10.3866/PKU.DXHX202309046
8. [8]
  Zhongrui Wang , Yuwen Meng , Xu Wang . 双层水凝胶的制备及其pH响应变形实验. University Chemistry, 2025, 40(8): 255-264. doi: 10.12461/PKU.DXHX202410038
9. [9]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
10. [10]
  Dongxue Han , Huiliang Sun , Li Niu . Virtual Reality Technology for Safe and Green University Chemistry Experimental Education. University Chemistry, 2024, 39(8): 191-196. doi: 10.3866/PKU.DXHX202312055
11. [11]
  Zhaoxuan ZHU , Lixin WANG , Xiaoning TANG , Long LI , Yan SHI , Jiaojing SHAO . Application of poly(vinyl alcohol) conductive hydrogel electrolytes in zinc ion batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 893-902. doi: 10.11862/CJIC.20240368
12. [12]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
13. [13]
  Hongyun Liu , Jiarun Li , Xinyi Li , Zhe Liu , Jiaxuan Li , Cong Xiao . Course Ideological and Political Design of a Comprehensive Chemistry Experiment: Constructing a Visual Molecular Logic System Based on Intelligent Hydrogel Film Electrodes. University Chemistry, 2024, 39(2): 227-233. doi: 10.3866/PKU.DXHX202309070
14. [14]
  Lijuan Liu , Xionglei Wang . Preparation of Hydrogels from Waste Thermosetting Unsaturated Polyester Resin by Controllable Catalytic Degradation: A Comprehensive Chemical Experiment. University Chemistry, 2024, 39(11): 313-318. doi: 10.12461/PKU.DXHX202403060
15. [15]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
16. [16]
  Leyuan Sun , Xiaoyu Xie , Fangfang Chen . 敦煌壁画的“DNA变身”. University Chemistry, 2025, 40(8): 211-217. doi: 10.12461/PKU.DXHX202410079
17. [17]
  Simin Fang , Hong Wu , Sizhe Sheng , Lingling Li , Yuxi Wang , Hongchun Li , Jun Jiang . The Food Kingdom Lecture Series: The Science behind Color. University Chemistry, 2024, 39(9): 177-182. doi: 10.12461/PKU.DXHX202402012
18. [18]
  Linghua Chen . 基于双联动“三学”模式的食品专业分析化学教学改革. University Chemistry, 2025, 40(8): 78-91. doi: 10.12461/PKU.DXHX202409095
19. [19]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
20. [20]
  Haifeng Liu , Yong Xiao , Teng Yuan , Bimin Lin , Yizhen Wang , Hui Zeng . Exploration of Safety Facility Configuration in University Chemical Depot. University Chemistry, 2024, 39(10): 182-188. doi: 10.3866/PKU.DXHX202401036

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(1517)
HTML views(283)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142