Citation: LIU Lu-Ying, LIU Jin-Hui, HU Xiao-Gang. Dispersive Solid-Phase Extraction Technology Based on Aptamer Modified Composite Nanofibers and Its Application in Detection of Ochratoxin A[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2096-2105. doi: 10.19756/j.issn.0253-3820.201728 shu

Dispersive Solid-Phase Extraction Technology Based on Aptamer Modified Composite Nanofibers and Its Application in Detection of Ochratoxin A

LIU Lu-Ying ^1,2 ,
LIU Jin-Hui ^2,3 ,
HU Xiao-Gang ^2,,

1.
Department of Chemistry, Guangdong University of Education, Guangzhou 510303, China;
2.
School of Chemistry, South China Normal University, Guangzhou 510631, China;
3.
Analysis & Testing Center, South China Normal University, Guangzhou 510631, China

Corresponding author: HU Xiao-Gang, huxg@scnu.edu.cn
Received Date: 4 December 2020
Revised Date: 17 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.21775048, 21275058), the Natural Science Foundation of Guangdong Province (No.2017A030313060), the Science and Technology Program of Guangzhou (No.2013B091500093), the Science and Technology Program of Guangzhou (No.201904010166) and Youth Innovation Talents Project of Colleges and Universities in Guangdong Province,China(No.2018KQNCX178).

The chitosan-sodium alginate composite nanofibers were prepared by freeze-drying. The nanofibers had a uniform filamentous structure with a width of about 0.4 μm. The aptamer of ochratoxin A was immobilized on the surface of the fiber by chemical bonding to obtain the aptamer-modified chitosan-sodium alginate composite nanofiber, and the bonding amount of the aptamer could reach 2.3 μg/mg. It could be used as an adsorbent for dispersive solid phase extraction technology. The adsorbent showed good extraction ability and high selectivity for ochratoxin A, the extraction capacity was about 3.1 ng/mg, and the extraction amount was 2.44-12.8 times of the structural analogue ochratoxin B and the five reference molecules. Compared with the composite nanofiber modified by the scrambled oligonucleotide and composite nanofiber, the extraction amount of ochratoxin A was increased by 4.88 and 13.0 times, respectively. Under the optimal extraction conditions, an analytical method based on aptamer modified composite nanofiber dispersion solid phase extraction-high performance liquid chromatography for determination of ochratoxin A was established. The linear range was 0.05-3.0 μg/L, the detection limit was 13 ng/L (S/N=3), and the recoveries of standard addition were 86.7%-101.0%. This method had good selectivity and high sensitivity, and could be applied to the detection of trace ochratoxin A in peanut, corn and wheat samples.
- Composite nanofibers,
- Aptamer,
- Dispersive solid-phase extraction,
- Ochratoxin A
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