Citation: LI Juan, LI Yan-Hua. Selective Enrichment of Glycopeptides Using Two-Dimentional Novel Nitrogenated Graphene Materials[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1555-1562,1596. doi: 10.19756/j.issn.0253-3820.201724 shu

Selective Enrichment of Glycopeptides Using Two-Dimentional Novel Nitrogenated Graphene Materials

LI Juan ,
LI Yan-Hua ^,

The Second Hospital Affiliated to Dalian Medical University, Dalian 116023, China

Corresponding author: LI Yan-Hua, m17709870057_1@163.com
Received Date: 3 December 2020
Revised Date: 6 July 2021

Fund Project: Supported by the Joint Fund Youth Project of the 2018 "Collaborative Innovation Center for Individualized Diagnosis and Treatment" of the Second Hospital Affiliated to Dalian Medical University (No.dy2yhws201807).

Isolation and identification of glycoproteins are of great significance for the study of their biological functions, the discovery of more biomarkers and the development of new biological drugs. However, due to the complex composition of biological samples and the low abundance of glycoproteins, it is very challenging to isolate and identify glycoproteins. Therefore, it is very important to develop an efficient method for glycopeptides enrichment. Nitrogen carbamide (C2N), a novel two-dimensional (2D) material, has high specific surface area, good hydrophilicity and abundant nitrogen, which provides the possibility of glycopeptides enrichment. In this study, a novel 2D C2N material was prepared for selective enrichment of glycopeptides. The pore size of the synthesized C2N was 2.5 nm and the specific surface area was 633 m²/g. The enrichment method based on C2N showed many advantages such as high selectivity (glycopeptides could be selectively enriched from the digests of bovine fetuin even mixed with the 200 mass folds of bovine serum albumin), high adsorption capacity (150 mg/g) and high recovery (82.2%±7.0%). The method was used for analysis of glycopeptide in 100 μg of HeLa cell lysates using three technical replicates and a total of 339, 353 and 327 glycosylation sites resulting from 328, 320 and 316 glycopeptides were characterized, respectively. This study provided a new technique for glycoproteomic and a methodological basis for the discovery of potential tumor markers.
- Nitrogenated graphene,
- Glycopeptides,
- Mass spectrometry,
- Enrichment
1. [1]
  SEELING M, BRVCKNER C, NIMMERJAHN F. Nat. Rev. Rheumatol., 2017, 13(10):621-630.
2. [2]
  RODRÍGUEZ E, SCHETTERS S T T, KOOYK Y. Nat. Rev. Immunol., 2018, 18(3):204-211.
3. [3]
  PINHO S S, REIS C A. Nat. Rev. Cancer, 2015, 15(9):540-555.
4. [4]
  VERHELST X, DIAS A M, COLOMBEL J F,VERMEIRE S, PINHO S S. Gastroenterology, 2020, 158(1):95-110.
5. [5]
  NARIMATSU H, KAJI H, VAKHRUSHEV S Y, CLAUSEN H, ZHANG H, NORO E, TOGAYACHI A, NAGAI-OKATANI C, KUNO A, ZOU X, CHENG L, TAO S C, SUN Y. J. Proteome. Res., 2018, 17(12):4097-4112.
6. [6]
7. [7]
  BIE Z, XING R, HE X, MA Y, CHEN Y, LIU Z. Anal. Chem., 2018, 90(16):9845-9852.
8. [8]
  CHEN J, SHAH P, ZHANG H. Anal. Chem., 2013, 85(22):10670-10674.
9. [9]
10. [10]
11. [11]
12. [12]
  QING G, YAN J Y, HE X N, LI X L, LIANG X M. TrAC-Trends Anal. Chem., 2020, 124:115570.
13. [13]
  LIU J, WANG F J, ZHU J, MAO J W, LIU Z Y, CHENG K, QIN H Q, ZOU H F. Anal. Bioanal. Chem., 2014, 406(13):3103-3109.
14. [14]
  CHEN R, STUPAK J, WILLIAMSON S, TWINE S M, LI J. Rapid Commun. Mass Spectrom., 2019, 33(15):1240-1247.
15. [15]
  GELINAS R, MAILLEUX F, DONTAINE J, BULTOT L, DEMEULDER B, GINION A, DASKALOPOULOS E P, ESFAHANI H, DUBOIS-DERUY E, LAUZIER B, GAUTHIER C, OLSON A K, BOUCHARD B, ROSIERS D C, VIOLLET B, SAKAMOTO K, BALLIGAND J L, VANOVERSCHELDE J L, BEAULOYE C, HORMAN S, BERTRAND L. Nat. Commun., 2015, 6:6486.
16. [16]
  ZHU L, XUE Q Z, LI X F, WUT T, JIN Y K, XING W. J. Mater. Chem. A, 2015, 3(42):21351-21356.
17. [17]
  YAR M, HASHMI M A, AYUB K. J. Mol. Liq., 2019, 296:111929.
18. [18]
  XU J, MAHMOOD T J, DOU Y H, DOU S X, LI F, DAI L M, BAEK J B. Adv. Mater., 2017, 29(34):1702007.
19. [19]
  DENG Z Z, YE M L, BIAN Y Y, LIU Z Y, LIU F J, WANG C L, QIN H Q, ZOU H F. Chem. Commun., 2014, 50(90):13960-13962.
1. [1]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
2. [2]
  Yunting Shang , Yue Dai , Jianxin Zhang , Nan Zhu , Yan Su . Something about RGO (Reduced Graphene Oxide). University Chemistry, 2024, 39(9): 273-278. doi: 10.3866/PKU.DXHX202306050
3. [3]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
4. [4]
  Zhuo WANG , Junshan ZHANG , Shaoyan YANG , Lingyan ZHOU , Yedi LI , Yuanpei LAN . Preparation and photocatalytic performance of CeO₂-reduced graphene oxide by thermal decomposition. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1708-1718. doi: 10.11862/CJIC.20240067
5. [5]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
6. [6]
  Yue Zhang , Bao Li , Lixin Wu . GO-Assisted Supramolecular Framework Membrane for High-Performance Separation of Nanosized Oil-in-Water Emulsions. Acta Physico-Chimica Sinica, 2024, 40(5): 2305038-0. doi: 10.3866/PKU.WHXB202305038
7. [7]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
8. [8]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
9. [9]
  Anbang Du , Yuanfan Wang , Zhihong Wei , Dongxu Zhang , Li Li , Weiqing Yang , Qianlu Sun , Lili Zhao , Weigao Xu , Yuxi Tian . Photothermal Microscopy of Graphene Flakes with Different Thicknesses. Acta Physico-Chimica Sinica, 2024, 40(5): 2304027-0. doi: 10.3866/PKU.WHXB202304027
10. [10]
  Zhenlin Zhou , Siyuan Chen , Yi Liu , Chengguo Hu , Faqiong Zhao . A New Program of Voltammetry Experiment Teaching Based on Laser-Scribed Graphene Electrode. University Chemistry, 2024, 39(2): 358-370. doi: 10.3866/PKU.DXHX202308049
11. [11]
  Jiahao Lu , Xin Ming , Yingjun Liu , Yuanyuan Hao , Peijuan Zhang , Songhan Shi , Yi Mao , Yue Yu , Shengying Cai , Zhen Xu , Chao Gao . High-Precision and Reliable Thermal Conductivity Measurement for Graphene Films Based on an Improved Steady-State Electric Heating Method. Acta Physico-Chimica Sinica, 2025, 41(5): 100045-0. doi: 10.1016/j.actphy.2025.100045
12. [12]
  Tianqi Bai , Kun Huang , Fachen Liu , Ruochen Shi , Wencai Ren , Songfeng Pei , Peng Gao , Zhongfan Liu . Nanoscale Mechanism of Microstructure-Dependent Thermal Diffusivity in Thick Graphene Sheets. Acta Physico-Chimica Sinica, 2025, 41(3): 100025-0. doi: 10.3866/PKU.WHXB202404024
13. [13]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
14. [14]
  Hailang JIA , Yujie LU , Pengcheng JI . Preparation and properties of nitrogen and phosphorus co-doped graphene carbon aerogel supported ruthenium electrocatalyst for hydrogen evolution reaction. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2327-2336. doi: 10.11862/CJIC.20250021
15. [15]
  Yuanchun Pan , Xinyun Lin , Leyi Yang , Wenya Hu , Dekui Song , Nan Liu . Artificial Intelligence Science Practice: Preparation of Electronic Skin by Chemical Vapor Deposition of Graphene. University Chemistry, 2025, 40(11): 272-280. doi: 10.12461/PKU.DXHX202412052
16. [16]
  Jiayao Wang , Guixu Pan , Ning Wang , Shihan Wang , Yaolin Zhu , Yunfeng Li . Preparation of donor-π-acceptor type graphitic carbon nitride photocatalytic systems via molecular level regulation for high-efficient H₂O₂ production. Acta Physico-Chimica Sinica, 2025, 41(12): 100168-0. doi: 10.1016/j.actphy.2025.100168
17. [17]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
18. [18]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
19. [19]
  Lisha LEI , Wei YONG , Yiting CHENG , Yibo WANG , Wenchao HUANG , Junhuan ZHAO , Zhongjie ZHAI , Yangbin DING . Application of regenerated cellulose and reduced graphene oxide film in synergistic power generation from moisture electricity generation and Mg-air batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1151-1161. doi: 10.11862/CJIC.20240202
20. [20]
  Chaolin Mi , Yuying Qin , Xinli Huang , Yijie Luo , Zhiwei Zhang , Chengxiang Wang , Yuanchang Shi , Longwei Yin , Rutao Wang . Galvanic Replacement Synthesis of Graphene Coupled Amorphous Antimony Nanoparticles for High-Performance Sodium-Ion Capacitor. Acta Physico-Chimica Sinica, 2024, 40(5): 2306011-0. doi: 10.3866/PKU.WHXB202306011

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(977)
HTML views(143)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142