Citation: LI Juan, LI Yan-Hua. Selective Enrichment of Glycopeptides Using Two-Dimentional Novel Nitrogenated Graphene Materials[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1555-1562,1596. doi: 10.19756/j.issn.0253-3820.201724 shu

Selective Enrichment of Glycopeptides Using Two-Dimentional Novel Nitrogenated Graphene Materials

LI Juan ,
LI Yan-Hua ^,

The Second Hospital Affiliated to Dalian Medical University, Dalian 116023, China

Corresponding author: LI Yan-Hua, m17709870057_1@163.com
Received Date: 3 December 2020
Revised Date: 6 July 2021

Fund Project: Supported by the Joint Fund Youth Project of the 2018 "Collaborative Innovation Center for Individualized Diagnosis and Treatment" of the Second Hospital Affiliated to Dalian Medical University (No.dy2yhws201807).

Isolation and identification of glycoproteins are of great significance for the study of their biological functions, the discovery of more biomarkers and the development of new biological drugs. However, due to the complex composition of biological samples and the low abundance of glycoproteins, it is very challenging to isolate and identify glycoproteins. Therefore, it is very important to develop an efficient method for glycopeptides enrichment. Nitrogen carbamide (C2N), a novel two-dimensional (2D) material, has high specific surface area, good hydrophilicity and abundant nitrogen, which provides the possibility of glycopeptides enrichment. In this study, a novel 2D C2N material was prepared for selective enrichment of glycopeptides. The pore size of the synthesized C2N was 2.5 nm and the specific surface area was 633 m²/g. The enrichment method based on C2N showed many advantages such as high selectivity (glycopeptides could be selectively enriched from the digests of bovine fetuin even mixed with the 200 mass folds of bovine serum albumin), high adsorption capacity (150 mg/g) and high recovery (82.2%±7.0%). The method was used for analysis of glycopeptide in 100 μg of HeLa cell lysates using three technical replicates and a total of 339, 353 and 327 glycosylation sites resulting from 328, 320 and 316 glycopeptides were characterized, respectively. This study provided a new technique for glycoproteomic and a methodological basis for the discovery of potential tumor markers.
- Nitrogenated graphene,
- Glycopeptides,
- Mass spectrometry,
- Enrichment
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