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Citation: LI Wan-Ming, FANG Jin. Application of Aptamer-functionalized Magnetic Beads in Enrichment of Metastatic Colorectal Cancer Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(5): 710-717. doi: 10.19756/j.issn.0253-3820.201717 shu

Application of Aptamer-functionalized Magnetic Beads in Enrichment of Metastatic Colorectal Cancer Cells

LI Wan-Ming ,
FANG Jin ^,

Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, Department of Cell Biology, School of Life Sciences, China Medical University, Shenyang 110122, China

Corresponding author: FANG Jin, jfang@cmu.edu.cn
Received Date: 30 November 2020
Revised Date: 4 January 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 81672920, 82073291), the Natural Science Foundation of Liaoning Province, China (No. 2020-MS-164) and the Shenyang Youth Science and Technology Project (No. RC190261).

Based on the high affinity and specific target recognition function of the aptamer for metastatic tumor cells, aptamer-functionalized magnetic beads (W3-beads) that could specifically recognize and enrich metastatic colorectal cancer cells were formed using the biotin-streptavidin cross-linking method. Aptamer W3 maintained specific binding ability to target cells at room temperature and in human plasma environment. W3-beads prepared by modifying magnetic beads effectively captured target cells in mixed cells of target cells/non-target cells in different ratios. When the number of target cells exceeded 10³ per milliliter, the capture efficiency of W3-beads exceeded 75%, and the actual detection limit reached 50 cells per milliliter. Besides, the calibration formula between the initial number of cells and the number of captured cells was y=0.7253x-5.1199 (R²=0.9967). Incorporating target cells into whole blood to simulate circulating tumor cells, W3-beads effectively captured and enriched target cells in whole blood. The aptamer-functioned magnetic beads constructed in this experiment had good targeted capture, separation and enrichment capabilities for metastatic colorectal cancer cells, and provided an effective and convenient tool for enrichment of circulating tumor cells in the blood samples of colorectal cancer patients.
- Circulating tumor cell,
- Aptamer,
- Magnetic beads,
- Enrichment,
- Metastatic colorectal cancer cell
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