Citation: FAN Zi-Yan, LIU Zheng-Jie, ZHANG Rui-Long, HAN Guang-Mei, ZHANG Zhong-Ping. Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(7): 1208-1217. doi: 10.19756/j.issn.0253-3820.201701 shu

Preparation of Lysosome-targeting Carbon Dots and Its Application in Cell Imaging

1.
Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China;
2.
Key Laboratory of Photovoltaic and Energy Conservation Materials, Institute of Solid State Physics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China

Corresponding author: HAN Guang-Mei, gmhan@ahu.edu.cn
Received Date: 24 November 2020
Revised Date: 26 April 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 22074001, 21874137, 21974001, 21775001) and the Science and Technology Service Network Initiative of Chinese Academy of Sciences (No. KFJ-STS-ZDTP-080).

The lysosome-targeting carbon dots with excellent water solubility and strong blue emission were synthesized through a one-step functional modification for long-term lysosome imaging in living cells. Based on the carboxyl rich carbon dots synthesized by oxidizing carbon black in refluxing HNO₃, N,N-dimethylethylenediamine (DMEDA) was chemically linked onto the carboxyl rich carbon dots through acylation reaction to obtain DMEDA modified carbon dots (M-CDs). The infrared spectrum of M-CDs showed the characteristic peak of acylamide (O=C-NH-) at 1658 cm^–1, indicating the successful modification of DMEDA. The quantum yield of M-CDs was 15.6%, which was about 27 times higher than the original carboxyl rich carbon dots, meeting the requirement of cell imaging. The probe entered cells through temperature-dependent endocytosis way and the co-localization experiment with commercial dye Lyso-Tracker red showed that the probe localized in lysosomes and could be used as a universal lysosome tracker in different cell lines. Moreover, compared to commercially available Lyso-Tracker blue, M-CDs were more photostable under UV light. The fluorescence of M-CDs was still brightness after time-lapsed imaging by confocal microscopy for 120 min with an interval of 5 min in living cells, implying that the M-CDs could be used for long-term imaging of lysosomes.
- Carbon dots,
- Functional modification,
- N,N-Dimethylethylenediamine,
- Lysosome target,
- Cell imaging
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