Citation: LI Hai-Yu, LI Wen-Ya, WANG Wan, LYU Qing, WANG Zhi-Juan, ZHANG Qing. Construction of Electrochemical Aptasensor Based on Sandwich Carbon Nanotube/Gold Film for Detection of Bisphenol A[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(3): 415-423. doi: 10.19756/j.issn.0253-3820.201465 shu

Construction of Electrochemical Aptasensor Based on Sandwich Carbon Nanotube/Gold Film for Detection of Bisphenol A

Chinese Academy of Inspection and Quarantine, Beijing 100176, China

Corresponding author: ZHANG Qing, njuzhangqing@caiq.org.cn
Received Date: 5 August 2020
Revised Date: 12 January 2021

Fund Project: National Key Research and Development Project of China (No. 2016YFF0203703) and the Industrial and Consumer Goods Quality Safety Assurance Project of SAMR.

An electrochemical aptasensor for detection of bisphenol A (BPA) was developed in this work. Disposable gold-film working electrode was modified with gold-coated multi-walled carbon nanotubes to form a sandwich structure, which could greatly enhance the surface area of the working electrode and electronic transport. TMB (3,3',5,5'-tetramethylbenzidine), acting as signal molecule, was intercalated into the immobilized aptamer to form a ssDNA-TMB complex. In the presence of BPA, the immobilized aptamer would have a conformational change, leading the intercalated TMB to be released from the complex. As a result, the electrochemical signal of the intercalated TMB was decreased. The change of peak current measured using differential pulse voltammetry was linearly correlated with the natural logarithm of concentration of BPA from 0.05 nmol/L to 500 nmol/L. The limit of detection was 43 pmol/L. The recoveries in real samples at different spiked levels were in the range of 88.9%-110.2%, and the relative standard deviations (RSDs) were 2.7%-9.0%. The proposed aptasensor here was simple, efficient and suitable for field testing, showing good potential for the supervision of consumer products safety.
- Carbon nanotube composites,
- 3,3',5,5'-tetramethylbenzidine,
- Aptasensor,
- Bisphenol A
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