Citation: LU Jia-Qi, YE Tai, XU Fei, CAO Hui, YUAN Min, YU Jin-Song, YIN Feng-Qin, WU Xiu-Xiu. DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides

Shanghai Engineering Research Center for Food Rapid Detection, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: YE Tai, taiye@usst.edu.cn
Received Date: 27 July 2020
Revised Date: 30 December 2020

Fund Project: Supported by the National Natural Science Foundation of China (No. 31801636) and the Shanghai Sailing Program (No. 18YF1417300).

A self-powered DNA machine was constructed by loading DNAzyme strand and dye-labelled substrate strand co-modified gold nanoparticles (AuNPs) on MnO₂ nanosheets via van der Waals force. When acetylcholinesterase (AChE) converted its substrate, acetylthiocholine (ATCh), into thiocholine (TCh), the MnO₂ nanosheets were degraded into Mn²⁺. The Mn²⁺ dependent-DNAzyme was triggered and plenty of dye-labelled fragments were released from the surface of AuNPs, which would lead to the enhancement of fluorescence. When AChE was inhibited by organophosphorus pesticides (OPs), the DNA machine could not be activated. This self-powered DNA machine was characterized by transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy, zeta potential, dynamic light scattering (DLS) and X-ray photoelectron spectroscopy (XPS), and the performance of DNA machine was optimized. Under the optimal conditions, the proposed DNA machine was applied to the determination of dichlorvos (DDVP) with a linear range of 5-500 μg/L and a limit of detection of 3.62 μg/L (3σ). The method presented here was applied to the recovery assay of DDVP in spiked lettuce. The recoveries for DDVP were 96.5%-104.2%, and the relative standard deviations (RSD) were 1.7%-7.5%.
- Organophosphorus pesticide,
- DNA machine,
- MnO₂ nanosheets,
- DNAzyme
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
45. [45]
1. [1]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
2. [2]
  Junyuan Zhang , Zhiwei Miao . 有机磷杀虫剂的前世今生. University Chemistry, 2025, 40(6): 129-138. doi: 10.12461/PKU.DXHX202408118
3. [3]
  Qianlang Wang , Jijun Sun , Qian Chen , Quanqin Zhao , Baojuan Xi . The Appeal of Organophosphorus Compounds: Clearing Their Name. University Chemistry, 2025, 40(4): 299-306. doi: 10.12461/PKU.DXHX202405205
4. [4]
  Shiyang He , Dandan Chu , Zhixin Pang , Yuhang Du , Jiayi Wang , Yuhong Chen , Yumeng Su , Jianhua Qin , Xiangrong Pan , Zhan Zhou , Jingguo Li , Lufang Ma , Chaoliang Tan . 铂单原子功能化的二维Al-TCPP金属-有机框架纳米片用于增强光动力抗菌治疗. Acta Physico-Chimica Sinica, 2025, 41(5): 100046-. doi: 10.1016/j.actphy.2025.100046
5. [5]
  Jiahong ZHENG , Jingyun YANG . Preparation and electrochemical properties of hollow dodecahedral CoNi₂S₄ supported by MnO₂ nanowires. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1881-1891. doi: 10.11862/CJIC.20240170
6. [6]
  Shuai Yuan , Yaofeng Yuan . Academician Chengye Yuan and Organic Phosphorus Chemistry. University Chemistry, 2025, 40(7): 393-400. doi: 10.12461/PKU.DXHX202409123
7. [7]
  Shicheng Yan . Experimental Teaching Design for the Integration of Scientific Research and Teaching: A Case Study on Organic Electrooxidation. University Chemistry, 2024, 39(11): 350-358. doi: 10.12461/PKU.DXHX202408036
8. [8]
  Yiqian Jiang , Zihan Yang , Xiuru Bi , Nan Yao , Peiqing Zhao , Xu Meng . Mediated electron transfer process in α-MnO₂ catalyzed Fenton-like reaction for oxytetracycline degradation. Chinese Chemical Letters, 2024, 35(8): 109331-. doi: 10.1016/j.cclet.2023.109331
9. [9]
  Shilong Li , Ming Zhao , Yefei Xu , Zhanyi Liu , Mian Li , Qing Huang , Xiang Wu . Performance optimization of aqueous Zn/MnO₂ batteries through the synergistic effect of PVP intercalation and GO coating. Chinese Chemical Letters, 2025, 36(3): 110701-. doi: 10.1016/j.cclet.2024.110701
10. [10]
  Kun Chen , Huimin Lin , Xin Peng , Ziying Wu , Jingyue Dai , Yi Sun , Yaxuan Feng , Ziyi Huang , Zhiqiang Yu , Meng Yu , Guangyu Yao , Jigang Wang . In situ synthesis of MnO₂ micro/nano-adjuvants for enhanced immunotherapy of breast tumors. Chinese Chemical Letters, 2025, 36(5): 110045-. doi: 10.1016/j.cclet.2024.110045
11. [11]
  Haoting Wang , Mengfan Luo , Yuzhong Wang , Jialong Yin , Heng Zhang , Jia Zhao , Bo Lai . Mn(Ⅱ) enhanced permanganate oxidation of trace organic pollutants in water: Critical role of in situ formation of colloidal MnO₂. Chinese Chemical Letters, 2025, 36(6): 110348-. doi: 10.1016/j.cclet.2024.110348
12. [12]
  Dan Liu . 可见光-有机小分子协同催化的不对称自由基反应研究进展. University Chemistry, 2025, 40(6): 118-128. doi: 10.12461/PKU.DXHX202408101
13. [13]
  Qinjin DAI , Shan FAN , Pengyang FAN , Xiaoying ZHENG , Wei DONG , Mengxue WANG , Yong ZHANG . Performance of oxygen vacancy-rich V-doped MnO₂ for high-performance aqueous zinc ion battery. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 453-460. doi: 10.11862/CJIC.20240326
14. [14]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
15. [15]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
16. [16]
  Qiangqiang SUN , Pengcheng ZHAO , Ruoyu WU , Baoyue CAO . Multistage microporous bifunctional catalyst constructed by P-doped nickel-based sulfide ultra-thin nanosheets for energy-efficient hydrogen production from water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1151-1161. doi: 10.11862/CJIC.20230454
17. [17]
  Jiali CHEN , Guoxiang ZHAO , Yayu YAN , Wanting XIA , Qiaohong LI , Jian ZHANG . Machine learning exploring the adsorption of electronic gases on zeolite molecular sieves. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 155-164. doi: 10.11862/CJIC.20240408
18. [18]
  Xiaoling LUO , Pintian ZOU , Xiaoyan WANG , Zheng LIU , Xiangfei KONG , Qun TANG , Sheng WANG . Synthesis, crystal structures, and properties of lanthanide metal-organic frameworks based on 2, 5-dibromoterephthalic acid ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1143-1150. doi: 10.11862/CJIC.20230271
19. [19]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
20. [20]
  Jun LUO , Baoshu LIU , Yunchang ZHANG , Bingkai WANG , Beibei GUO , Lan SHE , Tianheng CHEN . Europium(Ⅲ) metal-organic framework as a fluorescent probe for selectively and sensitively sensing Pb²⁺ in aqueous solution. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2438-2444. doi: 10.11862/CJIC.20240240

Get Citation

PDF

XML

Metrics

PDF Downloads(23)
Abstract views(787)
HTML views(127)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142