Citation: LIU Fu-Xin, DU Shi-Long, LI Lin, LIU Qian, DING Lan, LIU Xiu-Hui. A Novel Electrochemical Sensor Based on Pt Nanoparticles/Carbon Nanospheres for Direct Detection of Peroxynitrite Anion Released by Living Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2086-2095. doi: 10.19756/j.issn.0253-3820.201398 shu

A Novel Electrochemical Sensor Based on Pt Nanoparticles/Carbon Nanospheres for Direct Detection of Peroxynitrite Anion Released by Living Cells

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China

Corresponding author: LIU Xiu-Hui, liuxh@nwnu.edu.cn
Received Date: 8 July 2020
Revised Date: 27 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21565021).

Peroxynitrite anion (ONOO^-) is one of the most important molecules in biological systems and is usually maintained at a very low concentration level under normal physiological condition. However, under oxidative stress, the body will produce high concentration of ONOO^- abnormally, which may damage many biomolecules seriously and affect the physiological functions of cells. Therefore, it is very important to construct a sensor that can detect ONOO^- released by cells sensitively and rapidly. In this work, a novel ONOO^- electrochemical sensor was constructed based on carbon nanospheres (CNS) composite materials modified with platinum nanoparticles (PtNPs). Electron microscopes and electrochemical technology were used for characterization of the prepared CTS/PtNPs/CNS. The sensor showed excellent analytical performance for ONOO^- detection, with a wide linear range from 0.615 nmol/L to 0.139 mmol/L, and an extremely low detection limit of 0.205 nmol/L (S/N=3). In addition, it also showed good selectivity, stability and reproducibility, and was applied to detect ONOO^- released from living cells. The released ONOO^- of cells induced by Cd²⁺ was investigated, and it was found that more ONOO^- released from cells at higher Cd²⁺ concentration. Finally, the protective effects of the α-lipoic acid (ALA) and glutathione (GSH) on cells were investigated by electrochemical method, and it was found that the antioxidants mixture (ALA+GSH) had better protect cells due to their synergistic effect. The experimental results showed that the developed sensing platform and efficient antioxidation strategy provided the possibility for the future application in the biomedical field and cancer diseases.
- Peroxynitrite anion,
- Electrochemical sensor,
- Carbon nanospheres,
- Antioxidant,
- Platinum nanoparticles,
- Living cell
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