Citation: ZHANG Yi, HAO Liang-Wen, SHEN Xuan-Ang, JIANG Hu, HUANG Xiao-Lin, XIONG Yong-Hua. Novel Magnet-coated Gold Nanoparticles-based Immunochromatographic Strip for Quantitative Detection of Human Chorionic Gonadotropin in Serum[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(12): 1666-1673. doi: 10.19756/j.issn.0253-3820.201316 shu

Novel Magnet-coated Gold Nanoparticles-based Immunochromatographic Strip for Quantitative Detection of Human Chorionic Gonadotropin in Serum

ZHANG Yi ¹ ,
HAO Liang-Wen ^2,3 ,
SHEN Xuan-Ang ¹ ,
JIANG Hu ^2,3 ,
HUANG Xiao-Lin ^1,2,3 ,
XIONG Yong-Hua ^1,2,3,,

1.
School of Food Science, Nanchang University, Nanchang 330031, China;
2.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
3.
Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China

Corresponding author: XIONG Yong-Hua, yhxiongchen@163.com
Received Date: 1 June 2020
Revised Date: 25 September 2020

Fund Project: This work was supported by the National Key Research and Development Program of China (No. 2018YFC1602505).

A facile emulsion self-assembly strategy by co-assembling oleylamine-coated gold nanoparticles (OA-AuNPs) with oleic acid-coated iron oxide nanoparticles (OC-IONPs) into polymer nanobeads to form magnetic coated gold nanoparticles (MGNPs) was reported. The synthesized MGNPs exhibited a typical "magnetic-coated gold" core-shell heterostructure. Due to that OA-AuNPs aggregated in core and OC-IONPs assembled in shell, the magnetic shielding effect of OA-AuNPs was effectively avoided. Therefore, compared with the traditional "gold-coated magnetic" nanostructures, the MGNPs synthesized here had higher magnetic saturation intensity (80% of OC-IONPs) and strong absorbance (12.5 times that of the traditional 30 nm colloidal gold nanoparticles). By using MGNPs as the dual-function labeled probe and human chorionic gonadotropin (HCG) as a model biomarker, a highly sensitive immunochromatographic method (MGNPs-ICA) for detecting biomarkers in human serum was established. The results showed that the linear range of the MGNPs-ICA strip for quantitative detection of HCG in human serum was 0.97-250 mIU/mL, and the linear regression equation was y=0.2561lnx-0.0429 (R²=0.9816). The detection limit was 0.97 mIU/mL. The intra-and inter-assay recoveries for HCG spiked serum were 93.7%-109.1%, and the coefficient of variation was less than 14.3%. The specific experimental results showed that the method only showed a significant signal response to target HCG. In addition, the results of this method were in good agreement with those of chemiluminescence immunoassay (CLIA). Compared with CLIA method, MGNPs-ICA method had significant advantages in detection time, cost and portability. In a word, the as-prepared MGNPs had excellent magnetic properties and strong optical sensing ability, and could significantly improve the detection performance of ICA platform.
- Magnetic coated gold nanoparticles,
- Immunochromatographic strip,
- Human chorionic gonadotropin,
- Quantitative detection
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