Citation: LI Hai-Yin, CHANG Jia-Fu, LYU Wen-Xin, LI Feng. Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(10): 1325-1333. doi: 10.19756/j.issn.0253-3820.201295 shu

Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China

Received Date: 22 May 2020
Revised Date: 2 July 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21605093, 21775082).

A highly sensitive and label-free fluorescence carcinoembryonic antigen (CEA) biosensor was developed via target-triggered enzymatic recycling amplification reaction. In this strategy, aggregation induced emission fluorogen (AIEgen) TPE-M was chosen as a signal reporter to enhance the detection efficiency, as well as L-cysteine (L-cys) was utilized as a reactant to control over the fluorescence intensity due to its distinct capability of reacting with TPE-M and hemin/G-quadruplex. CEA triggered the in-situ generation of abundant hemin/G-quadruplex through polymerase/nicking enzyme-assisted recycling amplification, and the in-situ generated hemin/G-quadruplex catalyzed the destruction of L-cys. With L-cys consuming, a significantly decreased fluorescence was observed, demonstrating that the fluorescence intensity was relied on CEA amount. As a consequence, a facile, precise and sensitive strategy for CEA assay was readily realized. Furthermore, the detection limit was 0.033 fmol/L (S/N=3). The developed AIEgen-based biosensor provided a new method for sensitive and reliable detection of CEA in biological liquids, displaying a significant promise for CEA-related disease diagnosis.
- Aggregation induced emission,
- Label-free,
- Carcinoembryonic antigen,
- Fluorescence,
- Biosensor
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