Citation: WANG Jiang, HUANG Li-Juan, XU Zhen-Jun, LIU Yu, XU Jing-Juan, CHEN Hong-Yuan. Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(10): 1315-1324. doi: 10.19756/j.issn.0253-3820.201263 shu

Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry

1.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
2.
Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School Cardiothoracic Surgery, Nanjing 210008, China

Received Date: 8 May 2020
Revised Date: 24 June 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21327902, 21974060).

A custom membrane extraction electrospray ionization mass spectrometry (MEESI-MS) method was developed to detect the representative bacteric metabolites and classify four kinds of bacteria including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The chemical fingerprints of each bacterium were obtained using this MEESI-MS method. Principal component analysis (PCA) was then applied to analyze the information from the obtained fingerprint spectra and classify the samples. The results showed that MEESI-MS method was capable of rapidly obtaining metabolic fingerprints of blood samples and direct identifying the representative metabolites from each bacterium via employing both accurate mass measurements and structural information from tandem mass spectra. The overall analysis time was less than 65 min (including a culture time of about 60 min). This method was featured by the simplicity in sample pretreatment, ionization activation and data analysis and was promising in rapid identification of bacterial species in clinical bacteremia and septicemia.
- Membrane extraction electrospray ionization,
- Mass spectrometry,
- Principal component analysis,
- Bacteremia,
- Bloodstream infection
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沈阳化工大学材料科学与工程学院沈阳 110142