Citation: WANG Cun, LIU Fan-Xin, HUI Jun-Min, ZOU Xiao-Chuan, DENG Ming-Chuan, ZHENG Tian-Ping, LIU Lin. Biosensor Based on Methylene Blue Encapsulated Metal Organic Framework and Signal Amplification Strategy for Sensitive Detection of MicroRNA-141[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(9): 1185-1192. doi: 10.19756/j.issn.0253-3820.201222 shu

Biosensor Based on Methylene Blue Encapsulated Metal Organic Framework and Signal Amplification Strategy for Sensitive Detection of MicroRNA-141

Innovation Center for Lipid Resource and Children's Daily Chemicals, College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

Received Date: 22 April 2020
Revised Date: 13 July 2020

Fund Project: This work was supported by the Science and Technology Research Project of Chongqing Municipal Education Commission (No. KJQN201901604) and the Natural Science Foundation of Chongqing, China (No. cstc2018jcyjAX0110).

MicroRNA-141 (miRNA-141) is of great significance in the early diagnosis and treatment of related cancers. Here, a biosensor was developed for detection of miRNA-141. Firstly, bio-metal organic framework (bio-MOF) with a certain porosity and good biocompatibility was synthesized. The bio-MOF could encapsulate a large number of methylene blue (MB) via ion-exchange process and maintain the biological activity of MB. Secondly, as one of the target recycling signal amplification technologies, catalyzed hairpin assembly (CHA) could assist the target miRNA-141 cycle to achieve signal amplification and further improve the biosensor sensitivity. As a result, the proposed ECL biosensor exhibited good sensitivity for microRNA-141 detection in a linear range from 1.0×10^-16 mol/L to 1.0×10^-6 mol/L with a limit of detection (LOD) of 3.3×10^-17 mol/L (S/N=3). The spiked recoveries ranged from 99.9% to 102.0% (RSD ≤ 2.1%). The biosensor showed good stability and selectivity, wide linear range and high sensitivity, and opened up a new avenue for highly sensitive detection of various miRNAs.
- Biosensor,
- Catalyzed hairpin assembly,
- Signal amplification strategy,
- MicroRNA-141,
- Bio-metal organic framework,
- Methylene blue
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