Citation: YANG Mei, SHAO Ze-Yu, LIAO Xiao-Ling, XU Wen-Feng, ZHANG Yuan-Yuan. Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(12): 1674-1680. doi: 10.19756/j.issn.0253-3820.191705 shu

Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline

YANG Mei ^1,2 ,
SHAO Ze-Yu ^1,2 ,
LIAO Xiao-Ling ^1,2 ,
XU Wen-Feng ^1,2 ,
ZHANG Yuan-Yuan ^1,2

1.
Institute of Biomedical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China;
2.
Chongqing Engineering Laboratory of Nano/Micro Biological Medicine Detection Technology, Chongqing University of Science and Technology, Chongqing 401331, China

Received Date: 21 December 2019
Revised Date: 28 October 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 11532004), the Natural Science Foundation of Chongqing Science and Technology Commission (Nos. CSTC2018jcyjAX0286, CSTC2019JCYJ-msxmX0221, CSTC 2019JSCX-msxmX0231), and the Postgraduate Science and Technology Innovation Program of Chongqing University of Science and Technology (No. YKJCX1820206).

A novel antibacterial material of Ag nanoclusters hydrogel using silver nitrate and L-cysteine as raw materials was prepared by ultraviolet light reduction method. The structure and morphology of the material were characterized by transmission electron microscope (TEM), X-ray photoelectron spectra (XPS), scanning electron microscope (SEM) and X ray energy spectrum analysis (EDS). The antimicrobial properties of Ag nanoclusters hydrogel against sulfate reducing bacteria (SRB) were further studied through the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), growth curve and bacterial staining experiment using anaerobic workstations. The results indicated that, compared with the conventional inorganic antibacterial material sodium hypochlorite, the prepared Ag nanoclusters hydrogel had excellent antibacterial effect on SRB, and the MIC and MBC against SRB were 18.75 μg/mL and 37.5 μg/mL, respectively.
- Oilfield pipeline,
- Bacterial corrosion,
- Sulfate reducing bacteria,
- Ag nanoclusters hydrogel,
- Antibacterial properties
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