Citation: ZHAO Hong, WANG Yu-Ying, ZHAO Liang-Juan, MI Jie-Bo, ZHAO Hua-Bing, ZHANG Feng, FENG Feng, ZENG Jing, WANG Hong-Bin, DONG Zhi-Zhen. Targeted Analysis of Candidate Characteristic Peptides of Staphylococcus Aureus[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(7): 921-927. doi: 10.19756/j.issn.0253-3820.191703 shu

Targeted Analysis of Candidate Characteristic Peptides of Staphylococcus Aureus

1.
Animal, Plant and Food Stuffs Inspection Center of Tianjin Customs, Key Laboratory of Port Non-traditional Security Risk Prevention and Control, Tianjin 300461, China;
2.
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China;
3.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
4.
Beijing Customs District of the People's Republic China, Beijing 100026, China

Received Date: 29 November 2019
Revised Date: 22 May 2020

Fund Project: This work was supported by the National Key Research and Development Project Program of China (No.2017YFC1601600).

Staphylococcus aureus is a typical food-borne pathogenic bacterium. The detection methods for Staphylococcus aureus still have some shortcomings, and it is necessary to develop new and fast detection methods. In this work, a new method for detecting food-borne pathogenic bacteria through the detection of their characteristic peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. For the nine candidate characteristic peptides of Staphylococcus aureus reported in literature, it was necessary to investigate their targeted methods and compare their analysis performance. This study firstly investigated the untargeted method of nine candidate characteristic peptides, and then confirmed their targeted method based on multiple reaction monitoring model, after which their analysis performance were compared. The results indicated that I1 (AYLAVPAAPK), I2 (DYNSPTLIGWVK), I5 (AGYTVNNTPK) and I6 (SISSGYTSGR) were more suitable candidate characteristic peptides for Staphylococcus aureus. This work provided methodological basis for future application of detecting food-borne pathogenic bacteria through characteristic peptides.
- Food-borne pathogenic bacteria,
- Staphylococcus aureus,
- Characteristic peptides,
- Liquid chromatography-tandem mass spectrometry
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
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