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Citation: ZHAO Yi, SHI Xiao-Yu, SUN Hong, YAO Fu-Jun, KANG Xiao-Feng. Polyaniline Nanopores for DNA Analysis[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(3): 332-338. doi: 10.19756/j.issn.0253-3820.191697 shu

Polyaniline Nanopores for DNA Analysis

Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China

Received Date: 27 November 2019
Revised Date: 23 December 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21327806, 21874107)

Solid state nanopores are widely used as sensing elements for protein and nucleic acid analysis. However, the extremely low capture rate and fast translocation speed limit the sensitivity and resolution of nanopore-based sensors. In this work, we prepared a polyaniline conductive polymer-modified solid nanopore and explored its application for studying the translocation behavior of ssDNA and dsDNA. The results demonstrated that the electrostatic interaction between the polyaniline and DNA significantly reduced the translocation rate of ssDNA to 48.2 μs/base; meanwhile, the capture efficiency of the polyaniline nanopore for ssDNA was significantly increased by using LiCl as the electrolyte solution. Additional experimental results revealed that polyaniline nanopores could distinguish ssDNA and dsDNA based on the difference in blockage amplitude and translocation time. The results showed that functional modification of solid state nanopore could regulate DNA translocation behavior effectively. In addition, the polyaniline nanopores were expected to be used as a single-molecule nanodevice for the analysis and detection of biomolecules.
- Polyaniline,
- Solid state nanopore,
- Single ion track film,
- DNA analysis,
- Biological analysis
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