Citation: CUI Min, WEN Xiao-Fang, REN Ju-Jie, ZHANG Cong, LI Na, HAN Yuan-Yuan, YU Xu-Dong, JI Xue-Ping. Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(2): 224-232. doi: 10.19756/j.issn.0253-3820.191600 shu

Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide

CUI Min ^1, ,
WEN Xiao-Fang ¹ ,
REN Ju-Jie ¹ ,
ZHANG Cong ¹ ,
LI Na ¹ ,
HAN Yuan-Yuan ¹ ,
YU Xu-Dong ¹ ,
JI Xue-Ping ²

1.
Department of Chemistry, School of Sciences, Hebei University of Science and Technology, Shijiazhuang 050018, China;
2.
Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China

Received Date: 12 October 2019
Revised Date: 6 December 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81872669) and the Research Foundation of Education Department of Hebei Province of China (Nos. ZD2018037, QN2019140).

A non-enzymatic superoxide anion (O₂^·-) electrochemical sensor (Co₃[Fe(CN)₆]₂-RGO/GCE) was fabricated by co-depositing cobalt ferricyanide (Co₃[Fe(CN)₆]₂) prepared by coprecipitation method and reduced graphene oxide (RGO) prepared by Hummers method on a glassy carbon electrode (GCE). The characteristics of Co₃[Fe(CN)₆]₂-RGO nanocomposites and the possibility for electrochemical detection of O₂^·- were studied using scanning electron microscopy (SEM), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The mechanism of Co₃[Fe(CN)₆]₂-RGO for O₂^·- detection was investigated in this work. The sensor had a sensitivity of 91.8 μA L/(μmol cm²) and a detection limit of 0.071 μmol/L (S/N=3). Besides, it had excellent selectivity and could avoid the interference of hydrogen peroxide, oxygen and other electrochemical active substances in vivo. It was successfully applied to the electrochemical detection of superoxide anions in stimulated MCF-7 cells. The sensor had potential application for quantifying superoxide anions in the field of pathology and biology.
- Electrochemical sensor,
- Superoxide anion,
- Cobalt ferricyanide,
- Reduced graphene oxide,
- MCF-7 cells
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