Citation: GAO Xin, WAN Yao-Yao, LI Chang-Yin, DUAN Xu-Bin, DING Xuan-Sheng, JU Wen-Zheng. Systematic Screening and Assignment of Flavones in Total Flavones of Abelmoschus Manihot Based on High-performance Liquid Chromatography Time-of-Flight Mass Spectrometry Analysis and Mass Defect Filter[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(2): 262-268. doi: 10.19756/j.issn.0253-3820.191570 shu

Systematic Screening and Assignment of Flavones in Total Flavones of Abelmoschus Manihot Based on High-performance Liquid Chromatography Time-of-Flight Mass Spectrometry Analysis and Mass Defect Filter

1.
Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;
2.
School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China

Received Date: 25 September 2019
Revised Date: 20 December 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No.81503300).

High-performance liquid chromatography time-of-flight mass spectrometry (HPLC-TOF-MS) was employed to analyze the extract of total flavones of Abelmoschus manihot L. Medic (TFA) in both positive and negative ion mode. The raw LC-MS data were filtered by a well-established mass defect filter (MDF) approach to screen the flavone-related ions, and then, each screened ion was assigned by TOF-MS accurate mass measurement to certain elemental composition and ion type. Next, the co-eluted ions from one single compound were attempted to be combined, and the presence of each compound was confirmed by at least two quasi-molecular ions. Aided by authentic standards comparison and searching literature and databases, the targeted compounds were tentatively identified or assigned according to their parent and fragment ions. Finally, 49 flavones were screened and tentatively identified or assigned from the TFA extract. The results showed that, glycosylation, methylation, formylation, acetylation and glucuronidation were considered to be the major conversion involved in the formation of the TFA flavones. The current study not only provided the sufficient chemical substance for further studies of TFA, such as quality control and pharmacological activity, but also demonstrated the potential of the combination of LC-TOF-MS and MDF for systematically screening the constituents of herbal medicines.
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