Citation: WEN Xue, ZHENG Hai-Jiao, ZHANG Yang, JIA Qiong. Progress in Monolithic Column-based Separation and Enrichment of Glycoproteins[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 13-21. doi: 10.19756/j.issn.0253-3820.191566 shu

Progress in Monolithic Column-based Separation and Enrichment of Glycoproteins

1.
College of Pharmacy, Jilin University, Changchun 130033, China;
2.
College of Chemistry, Jilin University, Changchun 130012, China

Received Date: 21 September 2019
Revised Date: 23 October 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21575049).

As a post-translational modification, protein glycosylation not only reflects on body health, but also serves as the target of drug treatment. Therefore, it is of great significance to study protein glycosylation for the diagnosis and treatment of diseases. However, many glycoproteins of biological importance exhibit relatively low abundance, high complexity, and wide dynamic range in biological samples. It is indispensable to explore highly efficient sample pretreatment methods. In recent years, monolithic columns have attracted extensive attention in the field of separation and enrichment of glycoproteins due to their advantages of rapid separation, high efficiency, and good biological compatibility. In this paper, recent progress of monolithic columns in the field of separation and enrichment of glycoproteins is surveyed, and the future development is briefed.
- Monolithic column,
- Glycoprotein,
- Sample pretreatment,
- Separation,
- Enrichment,
- Review
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沈阳化工大学材料科学与工程学院沈阳 110142