Citation: WAN Jin-Xia, LI Yu-Long. Recent Advances in Detection Methods for Neurotransmitters[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(3): 307-315. doi: 10.19756/j.issn.0253-3820.191548 shu

Recent Advances in Detection Methods for Neurotransmitters

WAN Jin-Xia ^1,2, ,
LI Yu-Long ^1,2,3,4

1.
State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China;
2.
PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China;
3.
Peking-Tsinghua Center for Life Sciences, Beijing 100871, China;
4.
Chinese Institute for Brain Research, Beijing 100871, China

Received Date: 11 September 2019
Revised Date: 10 December 2019

Fund Project: This work was supported by the Beijing Municipal Science & Technology Commission (No. Z181100001318002), the National Basic Research Program of China (No. 2015CB856402), and the National Natural Science Foundation of China (Nos. 31671118, 31371442).

The human brain consists of billions of neurons, and the communication between neurons mainly relies on various neurotransmitters which are released at chemical synapses. Therefore, it is important to monitor neurotransmitter release with high temporal and spatial resolution to understand the functions of nervous system and in turn provide insights into psychiatric disease mechanisms. In this review, we summarize different categories of methods for monitoring neurotransmitters dynamics developed in recent decades in terms of principles, applications and limitations. We focus on the G protein-coupled receptor (GPCR) activation Based sensors, GRAB, which shows high temporal-spatial resolution, high sensitivity and high molecular specificity. GRAB sensors also perform well in multiple organisms, including zebrafish, drosophila and mice. In addition, the principle of GRAB sensors can be applied in general to develop sensors for detecting different neurotransmitters whose receptors share the conserved conformational changes when activated by corresponding ligands. In summary, these tools are useful for deciphering the dynamic regulation of a plethora of neurotransmitters in various neural circuits, and may enhance the understanding of the complex neuromodulation in both physiological and pathological processes.
- Neurotransmitters,
- Fluorescence imaging,
- G protein-coupled receptor,
- Review
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