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Citation: ZHOU Lu-Lu, CAI Ming-Jun, WANG Hui-Li, WANG Hong-Da. Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(2): 174-179. doi: 10.19756/j.issn.0253-3820.191502 shu

Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy

1.
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
University of Science and Technology of China, Hefei 230027, China

Received Date: 15 August 2019
Revised Date: 20 December 2019

Fund Project: This work was supported by the National Key R&D Program of China (No. 2017YFA0505300) and the National Natural Science Foundation of China (Nos. 21727816, 21721003).

A correlative super-resolution fluorescence and three-dimensional (3D) topography imaging microscopy was built, and the performance of the correlative system was evaluated with F-actin as an example. The experimental results suggested that the correlative instrument had the abilities to provide 3D overall topography and distribution of specific components at nanometer resolution, as well as to correlate the specific components' distribution with the overall topography. Thus, the correlative instrument is a powerful tool to investigate events regarding cytoskeleton and cellular substructure such as the composition and distribution characteristics of membrane protein clusters, and can also be widely applied to other fields such as life science and material science.
- Super-resolution fluorescence microscopy,
- Atomic force microscopy,
- Correlation
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