Citation: WANG Ying-Qi, CHEN Yao, CHEN Zeng-Ping. Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(2): 269-274. doi: 10.19756/j.issn.0253-3820.191400 shu

Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles

1.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
2.
Hunan Key Lab of Biomedical Materials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412008, China

Received Date: 13 July 2019
Revised Date: 3 December 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21705044, 21775038) and the Natural Science Foundation of Hunan Province, China (No. 2018JJ3114).

The core-shell nanoparticles (i.e., Au-core@4-mercaptobenzoic acid@Ag-shell) were combined with spectral shape deformation (SSD) quantitative model for quantification of 6-thioguanine (6-TG) in urine and red blood cell lysate samples. Experimental results showed that the combination of core-shell nanoparticles with SSD model could effectively remove the detrimental multiplicative effects on samples' surface enhanced Raman spectroscopy (SERS) signals caused by the variations of the number of "hot spots" and their distribution near/on the surfaces of SERS enhancing substrates, and hence realized the accurate quantitative determination of 6-TG in urine and red blood cell lysate samples with recovery of 95.6%-106.7%. The limit of detection (3σ) and limit of quantification (10σ) for 6-TG were estimated to be about 1 and 3 nmol/L, respectively. Compared with conventional methods for analysis of 6-TG such as high performance liquid chromatography (HPLC), the proposed method had many advantages such as simplicity, rapidity and high sensitivity, and had great potential in routine quantitative analysis of 6-TG in complex samples.
- Surface enhanced Raman spectroscopy,
- 6-Thioguanine,
- Core-shell nanoparticles,
- Spectral shape deformation quantitative theory
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